DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jee, Min Ki | ko |
dc.contributor.author | Kim, Ji Hoon | ko |
dc.contributor.author | Han, Yong Mahn | ko |
dc.contributor.author | Jung, Sung Jun | ko |
dc.contributor.author | Kang, Kyung Sun | ko |
dc.contributor.author | Kim, Dong Wook | ko |
dc.date.accessioned | 2013-03-09T19:39:00Z | - |
dc.date.available | 2013-03-09T19:39:00Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2010-02 | - |
dc.identifier.citation | PLOS ONE, v.5, no.2 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/10203/97319 | - |
dc.description.abstract | Background and Methods: In this study, we utilized a combination of low oxygen tension and a novel anti-oxidant, 4-(3,4-dihydroxy- phenyl)-derivative (DHP-d) to directly induce adipose tissue stromal cells (ATSC) to de-differentiate into more primitive stem cells. De-differentiated ATSCs was overexpress stemness genes, Rex-1, Oct-4, Sox-2, and Nanog. Additionally, demethylation of the regulatory regions of Rex-1, stemnesses, and HIF1 alpha and scavenging of reactive oxygen species were finally resulted in an improved stem cell behavior of de-differentiate ATSC (de-ATSC). Proliferation activity of ATSCs after dedifferentiation was induced by REX1, Oct4, and JAK/STAT3 directly or indirectly. De-ATSCs showed increased migration activity that mediated by P38/JUNK and ERK phosphorylation. Moreover, regenerative efficacy of de-ATSC engrafted spinal cord-injured rats and chemical-induced diabetes animals were significantly restored their functions. Conclusions/Significance: Our stem cell remodeling system may provide a good model which would provide insight into the molecular mechanisms underlying ATSC proliferation and transdifferentiation. Also, these multipotent stem cells can be harvested may provide us with a valuable reservoir of primitive and autologous stem cells for use in a broad spectrum of regenerative cell-based disease therapy. | - |
dc.language | English | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.subject | ACTIVATED PROTEIN-KINASES | - |
dc.subject | RADIAL GLIA | - |
dc.subject | STEM-CELL | - |
dc.subject | CEREBRAL-ISCHEMIA | - |
dc.subject | PLANT-CELLS | - |
dc.subject | ADULT-RATS | - |
dc.subject | DEDIFFERENTIATION | - |
dc.subject | PLASTICITY | - |
dc.subject | TRANSFORMATION | - |
dc.subject | IMPROVEMENT | - |
dc.title | DHP-Derivative and Low Oxygen Tension Effectively Induces Human Adipose Stromal Cell Reprogramming | - |
dc.type | Article | - |
dc.identifier.wosid | 000274442600001 | - |
dc.identifier.scopusid | 2-s2.0-77949361440 | - |
dc.type.rims | ART | - |
dc.citation.volume | 5 | - |
dc.citation.issue | 2 | - |
dc.citation.publicationname | PLOS ONE | - |
dc.identifier.doi | 10.1371/journal.pone.0009026 | - |
dc.contributor.localauthor | Han, Yong Mahn | - |
dc.contributor.nonIdAuthor | Jee, Min Ki | - |
dc.contributor.nonIdAuthor | Jung, Sung Jun | - |
dc.contributor.nonIdAuthor | Kang, Kyung Sun | - |
dc.contributor.nonIdAuthor | Kim, Dong Wook | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASES | - |
dc.subject.keywordPlus | RADIAL GLIA | - |
dc.subject.keywordPlus | STEM-CELL | - |
dc.subject.keywordPlus | CEREBRAL-ISCHEMIA | - |
dc.subject.keywordPlus | PLANT-CELLS | - |
dc.subject.keywordPlus | ADULT-RATS | - |
dc.subject.keywordPlus | DEDIFFERENTIATION | - |
dc.subject.keywordPlus | PLASTICITY | - |
dc.subject.keywordPlus | TRANSFORMATION | - |
dc.subject.keywordPlus | IMPROVEMENT | - |
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