DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yuk, Jae-Min | ko |
dc.contributor.author | Shin, Dong-Min | ko |
dc.contributor.author | Song, Kyoung-Sub | ko |
dc.contributor.author | Lim, Kyu | ko |
dc.contributor.author | Kim, Ki-Hye | ko |
dc.contributor.author | Lee, Sang-Hee | ko |
dc.contributor.author | Kim, Jin-Man | ko |
dc.contributor.author | Lee, Ji-Sook | ko |
dc.contributor.author | Paik, Tae-Hyun | ko |
dc.contributor.author | Kim, Jun-Sang | ko |
dc.contributor.author | Jo, Eun-Kyeong | ko |
dc.date.accessioned | 2013-03-09T19:11:58Z | - |
dc.date.available | 2013-03-09T19:11:58Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2010-01 | - |
dc.identifier.citation | AUTOPHAGY, v.6, no.1, pp.46 - 60 | - |
dc.identifier.issn | 1554-8627 | - |
dc.identifier.uri | http://hdl.handle.net/10203/97240 | - |
dc.description.abstract | The cell wall skeleton of Mycobacterium bovis Bacillus Calmette-Guerin (BCG/CWS) is an effective antitumor immunotherapy agent. Here, we demonstrate that BCG/CWS has a radiosensitizing effect on colon cancer cells through the induction of autophagic cell death. Exposure of HCT116 colon cancer cells to BCG/CWS before ionizing radiation (IR) resulted in increased cell death in a caspase-independent manner. Treatment with BCG/CWS plus IR resulted in the induction of autophagy in colon cancer cells. Either the autophagy inhibitor 3-methyladenine or knockdown of beclin 1 or Atg7 significantly reduced tumor cell death induced by BCG/CWS plus IR, whereas the caspase inhibitor z-VAD-fmk failed to do so. BCG/CWS plus IR-mediated autophagy and cell death was mediated predominantly by the generation of reactive oxygen species (ROS). The c-Jun NH(2)-terminal kinase pathway functioned upstream of ROS generation in the induction of autophagy and cell death in HCT116 cells after co-treatment with BCG/CWS and IR. Furthermore, toll-like receptor (TLR) 2, and in part, TLR4, were responsible for BCG/CWS-induced radiosensitization. In vivo studies revealed that BCG/ CWS-mediated radiosensitization of HCT116 xenograft growth is accompanied predominantly by autophagy. Our data suggest that BCG/CWS in combination with IR is a promising therapeutic strategy for enhancing radiation therapy in colon cancer cells through the induction of autophagy. | - |
dc.language | English | - |
dc.publisher | LANDES BIOSCIENCE | - |
dc.subject | RADIATION-INDUCED AUTOPHAGY | - |
dc.subject | ADVANCED RECTAL-CANCER | - |
dc.subject | PHASE-II TRIAL | - |
dc.subject | IMMUNE-RESPONSES | - |
dc.subject | DENDRITIC CELLS | - |
dc.subject | LUNG-CANCER | - |
dc.subject | DEATH | - |
dc.subject | SKELETON | - |
dc.subject | THERAPY | - |
dc.subject | IMMUNOTHERAPY | - |
dc.title | Bacillus calmette-guerin cell wall cytoskeleton enhances colon cancer radiosensitivity through autophagy | - |
dc.type | Article | - |
dc.identifier.wosid | 000276197900006 | - |
dc.identifier.scopusid | 2-s2.0-75149136162 | - |
dc.type.rims | ART | - |
dc.citation.volume | 6 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 46 | - |
dc.citation.endingpage | 60 | - |
dc.citation.publicationname | AUTOPHAGY | - |
dc.contributor.nonIdAuthor | Yuk, Jae-Min | - |
dc.contributor.nonIdAuthor | Shin, Dong-Min | - |
dc.contributor.nonIdAuthor | Song, Kyoung-Sub | - |
dc.contributor.nonIdAuthor | Lim, Kyu | - |
dc.contributor.nonIdAuthor | Kim, Ki-Hye | - |
dc.contributor.nonIdAuthor | Kim, Jin-Man | - |
dc.contributor.nonIdAuthor | Lee, Ji-Sook | - |
dc.contributor.nonIdAuthor | Paik, Tae-Hyun | - |
dc.contributor.nonIdAuthor | Kim, Jun-Sang | - |
dc.contributor.nonIdAuthor | Jo, Eun-Kyeong | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | BCG cell wall skeleton | - |
dc.subject.keywordAuthor | autophagy | - |
dc.subject.keywordAuthor | radiosensitization | - |
dc.subject.keywordAuthor | reactive oxygen species | - |
dc.subject.keywordAuthor | JNK | - |
dc.subject.keywordAuthor | toll-like receptor | - |
dc.subject.keywordPlus | RADIATION-INDUCED AUTOPHAGY | - |
dc.subject.keywordPlus | ADVANCED RECTAL-CANCER | - |
dc.subject.keywordPlus | PHASE-II TRIAL | - |
dc.subject.keywordPlus | IMMUNE-RESPONSES | - |
dc.subject.keywordPlus | DENDRITIC CELLS | - |
dc.subject.keywordPlus | LUNG-CANCER | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | SKELETON | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | IMMUNOTHERAPY | - |
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