Hydrophoretic high-throughput selection of platelets in physiological shear-stress range

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A gentle, but fast means for low-stress, high-throughput platelet purification is of significant clinical and biotechnological utility. Current implementations to sort platelets, however, require an external physical field, specialized buffer, or the harsh separation condition of high shear stress that tends to cause platelet stimulation. Here we report the use of hydrophoretic size separation in a wider channel and its parallelization to augment its throughput capability, maintaining physiological shear-stress range. We demonstrate a parallelized device comprising 10 stacks of the wide-channel hydrophoresis device, yielding a throughput of 2.9 million cells s(-1) and a platelet purity of 76.8%. The use of the wide channel for hydrophoresis also facilitates clogging-free separation by sorting blood clots and plaques. The wide-channel hydrophoresis offers the potential for gentle, fast, clogging-free sorting of rare blood cells with extreme throughput capabilities.
Publisher
ROYAL SOC CHEMISTRY
Issue Date
2011-02
Language
English
Article Type
Article
Citation

LAB ON A CHIP, v.11, no.3, pp.413 - 418

ISSN
1473-0197
DOI
10.1039/c0lc00148a
URI
http://hdl.handle.net/10203/97203
Appears in Collection
MSE-Journal Papers(저널논문)BiS-Journal Papers(저널논문)
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