Minimally invasive molecular delivery into the brain using optical modulation of vascular permeability

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Systemic delivery of bioactive molecules in the CNS is hampered by the blood-brain barrier, which has bottlenecked noninvasive physiological study of the brain and the development of CNS drugs. Here we report that irradiation with an ultrashort pulsed laser to the blood vessel wall induces transient leakage of blood plasma without compromising vascular integrity. By combining this method with a systemic injection, we delivered target molecules in various tissues, including the brain cortex. This tool allows minimally invasive local delivery of chemical probes, nanoparticles, and viral vectors into the brain cortex. Furthermore, we demonstrated astrocyte-mediated vasodilation in vivo without opening the skull, using this method to load a calcium indicator in conjunction with label-free photoactivation of astrocytes.
Publisher
NATL ACAD SCIENCES
Issue Date
2011-05
Language
English
Article Type
Article
Citation

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.108, no.22, pp.9256 - 9261

ISSN
0027-8424
DOI
10.1073/pnas.1018790108
URI
http://hdl.handle.net/10203/97201
Appears in Collection
MSE-Journal Papers(저널논문)BiS-Journal Papers(저널논문)
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