Unsorted human adipose tissue-derived stem cells promote angiogenesis and myogenesis in murine ischemic hindlimb model

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We examined the protective effect of unsorted human adipose tissue-derived stem cells (hADSCs) with a short-term culture in endothelial differentiation medium on tissue repair after ischemic injury. hADSCs were isolated from human subcutaneous adipose tissue and cultured in vitro in endothelial differentiation medium for 2 wks before transplantation. Cultured hADSCs showed a typical mesenchymal stromal cell-like phenotype, positive for endothelial-specific markers including VE-cadherin. Flt-1, eNOS, and vWF but not CD31. Two hours after ligation of the femoral artery and vein, mice were injected with the unselected hADSCs locally near the surgery site and tested for tissue perfusion and repair. Tissue perfusion rates of the ischemic limbs were significantly higher in the group treated with hADSCs compared with those of the control mice as early as post-operative day 3 (median 195.3%/min; interquartile range, 82.0-321.1 vs. median 47.1%/min; interquartile range, 18.0-58.7; p = 0.001 by Friedman two-way analysis). Subsequently, the mice treated with hADSC showed better prognosis at 4 wks after surgery, and the histological analysis revealed increased vascular density and reduced muscle atrophy in the hADSC-transplanted limbs. Moreover, hADSC-treated muscle contained differentiated myocytes positive for human NF-kappa B and myogenin antigen. These results collectively indicate that unsorted hADSCs after a 2-wk-in vitro culture have a therapeutic potential in ischemic tissue injury via inducing both angiogenesis and myogenesis. (C) 2010 Elsevier Inc. All rights reserved.
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Issue Date
2010-12
Language
English
Article Type
Article
Keywords

IMPROVE POSTNATAL NEOVASCULARIZATION; MESENCHYMAL STROMAL CELLS; SATELLITE CELLS; IN-VITRO; DIFFERENTIATION; TRANSPLANTATION; SECRETION; DISEASE; GROWTH; VIVO

Citation

MICROVASCULAR RESEARCH, v.80, pp.310 - 316

ISSN
0026-2862
DOI
10.1016/j.mvr.2010.05.006
URI
http://hdl.handle.net/10203/97185
Appears in Collection
BiS-Journal Papers(저널논문)
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