DC Field | Value | Language |
---|---|---|
dc.contributor.author | Manna, David | ko |
dc.contributor.author | Aligo, Jason | ko |
dc.contributor.author | Xu, Chenjia | ko |
dc.contributor.author | Park, Wei Sun | ko |
dc.contributor.author | Koc, Hasan | ko |
dc.contributor.author | Heo, Won Do | ko |
dc.contributor.author | Konan, Kouacou V. | ko |
dc.date.accessioned | 2013-03-09T17:10:47Z | - |
dc.date.available | 2013-03-09T17:10:47Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2010-03 | - |
dc.identifier.citation | VIROLOGY, v.398, no.1, pp.21 - 37 | - |
dc.identifier.issn | 0042-6822 | - |
dc.identifier.uri | http://hdl.handle.net/10203/96963 | - |
dc.description.abstract | During infection, hepatitis C virus (HCV) NS4B protein remodels host membranes to form HCV replication complexes (RC) which appear as foci under fluorescence microscopy (FM). To understand the role of Rab proteins in forming NS4B foci, cells expressing the HCV replicon were examined biochemically and via FM. First, we show that an isolated NS4B-bound subcellular fraction is competent for HCV RNA synthesis. Further, this fraction is differentially enriched in Rab1, 2, 5, 6 and 7. However, when examined via FM, NS4B foci appear to be selectively associated with Rab5 and Rab7 proteins. Additionally, dominant negative (DN) Rab6 expression impairs Rab5 recruitment into NS4B foci. Further, silencing of Rab5 or Rab7 resulted in a significant decrease in HCV genome replication. Finally, expression of DN Rab5 or Rab7 led to a reticular NS4B subcellular distribution, suggesting that endocytic proteins Rab5 and Rab7, but not Rab11, may facilitate NS4B foci formation. (C) 2009 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Endocytic Rab proteins are required for hepatitis C virus replication complex formation | - |
dc.type | Article | - |
dc.identifier.wosid | 000274669100003 | - |
dc.identifier.scopusid | 2-s2.0-76049106065 | - |
dc.type.rims | ART | - |
dc.citation.volume | 398 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 21 | - |
dc.citation.endingpage | 37 | - |
dc.citation.publicationname | VIROLOGY | - |
dc.identifier.doi | 10.1016/j.virol.2009.11.034 | - |
dc.contributor.localauthor | Heo, Won Do | - |
dc.contributor.nonIdAuthor | Manna, David | - |
dc.contributor.nonIdAuthor | Aligo, Jason | - |
dc.contributor.nonIdAuthor | Xu, Chenjia | - |
dc.contributor.nonIdAuthor | Park, Wei Sun | - |
dc.contributor.nonIdAuthor | Koc, Hasan | - |
dc.contributor.nonIdAuthor | Konan, Kouacou V. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | HCV NS4B | - |
dc.subject.keywordAuthor | NS4B FOCI | - |
dc.subject.keywordAuthor | HCV RC | - |
dc.subject.keywordAuthor | HCV RDRP Activity | - |
dc.subject.keywordAuthor | Membranous web | - |
dc.subject.keywordAuthor | Rab proteins | - |
dc.subject.keywordAuthor | Subcellular fractionation | - |
dc.subject.keywordAuthor | DN Rab proteins | - |
dc.subject.keywordAuthor | RNA silencing | - |
dc.subject.keywordPlus | HCV RNA REPLICATION | - |
dc.subject.keywordPlus | CELL-FREE SYSTEM | - |
dc.subject.keywordPlus | NONSTRUCTURAL PROTEIN | - |
dc.subject.keywordPlus | EARLY ENDOSOMES | - |
dc.subject.keywordPlus | SUBCELLULAR-LOCALIZATION | - |
dc.subject.keywordPlus | SUBGENOMIC REPLICON | - |
dc.subject.keywordPlus | BINDING PROTEIN | - |
dc.subject.keywordPlus | COPII VESICLES | - |
dc.subject.keywordPlus | TRANSPORT | - |
dc.subject.keywordPlus | GTPASE | - |
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