Strategies for efficient repetitive production of succinate using metabolically engineered Escherichia coli

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Escherichia coli AFP111, a pflB, ldhA, ptsG triple mutant of E. coli W1485, can be recovered for additional succinate production in fresh medium after two-stage fermentation (an aerobic growth stage followed by an anaerobic production stage). However, the specific productivity is lower than that of two-stage fermentation. In this study, three strategies were compared for reusing the cells. It was found when cells were aerobically cultivated at the end of two-stage fermentation without supplementing any carbon source, metabolites (mainly succinate and acetate) could be consumed. As a result, enzyme activities involved in the reductive arm of tricarboxylic acid cycle and the glyoxylate shunt were enhanced, yielding a succinate specific productivity above 125 mg g(DCW)(-1) h(-1) and a mass yield above 0.90 g g(-1) in the subsequent anaerobic fermentation. In addition, the intracellular NADH of cells subjected to aerobic cultivation with metabolites increased by more than 3.6 times and the ratio of NADH to NAD(+) increased from 0.4 to 1.3, which were both favorable for driving the TCA branch to succinate.
Publisher
Springer
Issue Date
2011-05
Language
English
Article Type
Article
Keywords

ACID PRODUCTION; LACTATE-DEHYDROGENASE; PYRUVATE-CARBOXYLASE; OVEREXPRESSION; FERMENTATIONS; GROWTH; MUTANT; SUCCINICIPRODUCENS; GLUCOSE; ENZYME

Citation

BIOPROCESS AND BIOSYSTEMS ENGINEERING, v.34, no.4, pp.411 - 418

ISSN
1615-7591
URI
http://hdl.handle.net/10203/95897
Appears in Collection
CBE-Journal Papers(저널논문)
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