Enhancement of xylitol production by attenuation of intracellular xylitol dehydrogenase activity in Candida tropicalis

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To construct Candida tropicalis strains that produce a high yield of xylitol with no requirement for co-substrates, we engineered the yeast with an attenuated xylitol dehydrogenase (XDH) and then assessed the efficiency of xylitol production The mutants, strains XDH-5 (with only one copy of the XDH gene), and ARSdR-16 (with a mutated XDH gene) showed 70 and 40% of wild type (WT) XDH activity, respectively. Conversions of xylose to xylitol by WT, XDH-5, and ARSdR-16 were 62, 64, and 75%, respectively, with productivities of 0.52, 0.54, and 0.62 g l(-1) h(-1), respectively. The ARSdR-16 mutant strain produced xylitol with high yield and high productivity in a simple process that required no co-substrates, such as glycerol. This strain represents a promising alternative for efficient and cost-effective xylitol production.
Publisher
SPRINGER
Issue Date
2011-06
Language
English
Article Type
Article
Keywords

GENE-DISRUPTED MUTANT; D-XYLOSE; YIELD

Citation

BIOTECHNOLOGY LETTERS, v.33, no.6, pp.1209 - 1213

ISSN
0141-5492
URI
http://hdl.handle.net/10203/95801
Appears in Collection
BS-Journal Papers(저널논문)
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