DC Field | Value | Language |
---|---|---|
dc.contributor.author | Luo, Sibin | ko |
dc.contributor.author | Cheng, Ru | ko |
dc.contributor.author | Meng, Fenghua | ko |
dc.contributor.author | Park, Tae Gwan | ko |
dc.contributor.author | Zhong, Zhiyuan | ko |
dc.date.accessioned | 2013-03-09T04:31:55Z | - |
dc.date.available | 2013-03-09T04:31:55Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-03 | - |
dc.identifier.citation | JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, v.49, no.15, pp.3366 - 3373 | - |
dc.identifier.issn | 0887-624X | - |
dc.identifier.uri | http://hdl.handle.net/10203/95367 | - |
dc.description.abstract | Water-soluble cationic polymers, poly(histamine acrylamide)s (PHAs), with superior buffer capacity at the endosomal pH range were designed, prepared, and investigated for non-viral gene transfection. PHAs were obtained with molecular weights ranging from 9.2 to 28.7 kDa through controlled radical polymerization of histamine acrylamide (HA). Acid-base titration results displayed that all PHA polymers had a remarkably high buffer capacity of about 70% at pH 5.1-7.2. 12.7-28.7 kDa PHAs were able to effectively condense DNA into nano-sized (< 220 nm) polyplexes with moderate positive surface charges (+13-+19 mV) at N/P ratios >= 10/1. CCK assays indicated that polyplexes of 12.7 and 17.5 kDa PHAs were nontoxic to COS-7 cells up to a tested N/P ratio of 20/1. Interestingly, the in vitro transfection using pCMV-Luc and pEGFP-C1 plasmid DNA as reporter genes showed that polyplexes of 12.7 kDa PHA formed at an N/P ratio of 20/1 mediated efficient transfection in COS-7 cells under 10% serum conditions, with transfection efficiencies comparable to that of 25 kDa polyethylenimine control. Their versatile design of structures, controlled synthesis, low cytotoxicity, and high transfection activity render PHA-based cationic polymers particularly interesting for the development of safe and efficient non-viral gene delivery systems. (C) 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 49: 3366-3373, 2011 | - |
dc.language | English | - |
dc.publisher | Wiley-Blackwell | - |
dc.subject | DELIVERY-SYSTEMS | - |
dc.subject | 2-(DIMETHYLAMINO)ETHYL METHACRYLATE | - |
dc.subject | DNA TRANSFECTION | - |
dc.subject | TRANSFER AGENTS | - |
dc.subject | POLYMERS | - |
dc.subject | COPOLYMERS | - |
dc.subject | POLYETHYLENIMINE | - |
dc.subject | HISTIDINE | - |
dc.subject | CELLS | - |
dc.subject | PH | - |
dc.title | Water Soluble Poly(histamine acrylamide) with Superior Buffer Capacity Mediates Efficient and Nontoxic In Vitro Gene Transfection | - |
dc.type | Article | - |
dc.identifier.wosid | 000293279500014 | - |
dc.identifier.scopusid | 2-s2.0-79959721610 | - |
dc.type.rims | ART | - |
dc.citation.volume | 49 | - |
dc.citation.issue | 15 | - |
dc.citation.beginningpage | 3366 | - |
dc.citation.endingpage | 3373 | - |
dc.citation.publicationname | JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY | - |
dc.contributor.localauthor | Park, Tae Gwan | - |
dc.contributor.nonIdAuthor | Luo, Sibin | - |
dc.contributor.nonIdAuthor | Cheng, Ru | - |
dc.contributor.nonIdAuthor | Meng, Fenghua | - |
dc.contributor.nonIdAuthor | Zhong, Zhiyuan | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | biological applications of polymers | - |
dc.subject.keywordAuthor | gene delivery | - |
dc.subject.keywordAuthor | imidazole | - |
dc.subject.keywordAuthor | plasmid DNA | - |
dc.subject.keywordAuthor | proton sponge effect | - |
dc.subject.keywordAuthor | radical polymerization | - |
dc.subject.keywordAuthor | water soluble polymers | - |
dc.subject.keywordPlus | DELIVERY-SYSTEMS | - |
dc.subject.keywordPlus | 2-(DIMETHYLAMINO)ETHYL METHACRYLATE | - |
dc.subject.keywordPlus | DNA TRANSFECTION | - |
dc.subject.keywordPlus | TRANSFER AGENTS | - |
dc.subject.keywordPlus | POLYMERS | - |
dc.subject.keywordPlus | COPOLYMERS | - |
dc.subject.keywordPlus | POLYETHYLENIMINE | - |
dc.subject.keywordPlus | HISTIDINE | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | PH | - |
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