Elucidating glycosaminoglycan-protein-protein interactions using carbohydrate microarray and computational approaches

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Glycosaminoglycan polysaccharides play critical roles in many cellular processes, ranging from viral invasion and angiogenesis to spinal cord injury. Their diverse biological activities are derived from an ability to regulate a remarkable number of proteins. However, few methods exist for the rapid identification of glycosaminoglycan-protein interactions and for studying the potential of glycosaminoglycans to assemble multimeric protein complexes. Here, we report a multidisciplinary approach that combines new carbohydrate microarray and computational modeling methodologies to elucidate glycosaminoglycan-protein interactions. The approach was validated through the study of known protein partners for heparan and chondroitin sulfate, including fibroblast growth factor 2 (FGF2) and its receptor FGFR1, the malarial protein VAR2CSA, and tumor necrosis factor-alpha (TNF-alpha). We also applied the approach to identify previously undescribed interactions between a specific sulfated epitope on chondroitin sulfate, CS-E, and the neurotrophins, a critical family of growth factors involved in the development, maintenance, and survival of the vertebrate nervous system. Our studies show for the first time that CS is capable of assembling multimeric signaling complexes and modulating neurotrophin signaling pathways. In addition, we identify a contiguous CS-E-binding site by computational modeling that suggests a potential mechanism to explain how CS may promote neurotrophin-tyrosine receptor kinase (Trk) complex formation and neurotrophin signaling. Together, our combined microarray and computational modeling methodologies provide a general, facile means to identify new glycosaminoglycan-protein-protein interactions, as well as a molecular-level understanding of those complexes.
Publisher
NATL ACAD SCIENCES
Issue Date
2011-06
Language
English
Article Type
Article
Keywords

FIBROBLAST-GROWTH-FACTOR; CHONDROITIN SULFATE; NEUROTROPHIN RECEPTORS; HIPPOCAMPAL-NEURONS; SIGNALING PATHWAYS; NEURITE OUTGROWTH; CRYSTAL-STRUCTURE; BINDING; HEPARIN; DIMERIZATION

Citation

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.108, no.24, pp.9747 - 9752

ISSN
0027-8424
DOI
10.1073/pnas.1102962108
URI
http://hdl.handle.net/10203/95320
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