DC Field | Value | Language |
---|---|---|
dc.contributor.author | Liu, Fei | ko |
dc.contributor.author | Choi, Ki-Seok | ko |
dc.contributor.author | Park, Tae-Jung | ko |
dc.contributor.author | Lee, Sang-Yup | ko |
dc.contributor.author | Seo, Tae-Seok | ko |
dc.date.accessioned | 2013-03-09T03:35:34Z | - |
dc.date.available | 2013-03-09T03:35:34Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-06 | - |
dc.identifier.citation | BIOCHIP JOURNAL, v.5, no.2, pp.123 - 128 | - |
dc.identifier.issn | 1976-0280 | - |
dc.identifier.uri | http://hdl.handle.net/10203/95260 | - |
dc.description.abstract | A graphene based biosensor system is presented for performing highly sensitive pathogenic virus detection. A free-standing conductive graphene film was prepared as a novel electrochemical sensor through two steps: synthesis of a graphene oxide (GO) film from GO colloidal suspensions by using a speed vacuum concentrator and thermal annealing process at 900 degrees C with H(2)/Ar flow to generate a reduced GO film. The resultant graphene film shows an excellent electron transfer property on the surface in the [Fe(CN)(6)](3-)/(4-) redox system and is used as a working electrode in the electrochemical biosensor. The surface of graphene is modified with pyrene derivatives, and then covalently linked with virus-specific antibodies. The target cell, rotavirus, is captured on the graphene film through antibody-antigen interaction, and the entire process was monitored by cyclic voltammetric responses. A 10(5) pfu/mL of input cells is detected with ca. 30.7% sensitivity, and ca. 1.3% sensitivity is measured with 10(3) pfu/mL of input cells, demonstrating that graphene film based electrode can be applied for electrochemical biosensor. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.title | Graphene-based electrochemical biosensor for pathogenic virus detection | - |
dc.type | Article | - |
dc.identifier.wosid | 000291665500004 | - |
dc.identifier.scopusid | 2-s2.0-79958794063 | - |
dc.type.rims | ART | - |
dc.citation.volume | 5 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 123 | - |
dc.citation.endingpage | 128 | - |
dc.citation.publicationname | BIOCHIP JOURNAL | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, Sang-Yup | - |
dc.contributor.localauthor | Seo, Tae-Seok | - |
dc.contributor.nonIdAuthor | Choi, Ki-Seok | - |
dc.contributor.nonIdAuthor | Park, Tae-Jung | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Graphene film | - |
dc.subject.keywordAuthor | Electrochemical biosensor | - |
dc.subject.keywordAuthor | Pathogen detection | - |
dc.subject.keywordAuthor | Detection sensitivity | - |
dc.subject.keywordPlus | CARBON NANOTUBES | - |
dc.subject.keywordPlus | FILMS | - |
dc.subject.keywordPlus | OXIDE | - |
dc.subject.keywordPlus | FLUORESCENCE | - |
dc.subject.keywordPlus | SHEETS | - |
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