DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, JH | ko |
dc.contributor.author | Kim, TY | ko |
dc.contributor.author | Lee, KH | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2013-03-09T02:42:12Z | - |
dc.date.available | 2013-03-09T02:42:12Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-04 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOENGINEERING, v.108, no.4, pp.934 - 946 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | http://hdl.handle.net/10203/95156 | - |
dc.description.abstract | We have previously reported the development of a 100% genetically defined engineered Escherichia coli strain capable of producing L-valine from glucose with a high yield of 0.38 g L-valine per gram glucose (0.58 mol L-valine per mol glucose) by batch culture. Here we report a systems biological strategy of employing flux response analysis in bioprocess development using L-valine production by fed-batch culture as an example. Through the systems-level analysis, the source of ATP was found to be important for efficient L-valine production. There existed a trade-off between L-valine production and biomass formation, which was optimized for the most efficient L-valine production. Furthermore, acetic acid feeding strategy was optimized based on flux response analysis. The final fed-batch cultivation strategy allowed production of 32.3 g/L L-valine, the highest concentration reported for E. coli. This approach of employing systems-level analysis of metabolic fluxes in developing fed-batch cultivation strategy would also be applicable in developing strategies for the efficient production of other bioproducts. Biotechnol. Bioeng. 2011;108: 934-946. (C) 2010 Wiley Periodicals, Inc. | - |
dc.language | English | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | Fed-Batch Culture of Escherichia coli for L-Valine Production Based on In Silico Flux Response Analysis | - |
dc.type | Article | - |
dc.identifier.wosid | 000288034700021 | - |
dc.identifier.scopusid | 2-s2.0-79952578981 | - |
dc.type.rims | ART | - |
dc.citation.volume | 108 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 934 | - |
dc.citation.endingpage | 946 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.identifier.doi | 10.1002/bit.22995 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Park, JH | - |
dc.contributor.nonIdAuthor | Kim, TY | - |
dc.contributor.nonIdAuthor | Lee, KH | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | L-valine | - |
dc.subject.keywordAuthor | fed-batch culture | - |
dc.subject.keywordAuthor | in silico flux response analysis | - |
dc.subject.keywordAuthor | systems metabolic engineering | - |
dc.subject.keywordPlus | CORYNEBACTERIUM-GLUTAMICUM | - |
dc.subject.keywordPlus | K-12 | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | MUTANT | - |
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