DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Ji-Eon | ko |
dc.contributor.author | Kim, Young-Kwan | ko |
dc.contributor.author | Min, Dal-Hee | ko |
dc.date.accessioned | 2013-03-09T02:22:56Z | - |
dc.date.available | 2013-03-09T02:22:56Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2010-10 | - |
dc.identifier.citation | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.132, no.42, pp.14714 - 14717 | - |
dc.identifier.issn | 0002-7863 | - |
dc.identifier.uri | http://hdl.handle.net/10203/95115 | - |
dc.description.abstract | A new method for quantitative phopholipase activity assays using mass spectrometry (MS) and a supported thin film consisting of a graphene oxide (GO)/carbon nanotube (CNT) double layer as a substrate for laser desorption ionization (LDI) has been developed. Phospholipids were very efficiently analyzed by LDI time-of-flight (TOF) MS on the GO/CNT films, presumably because of the affinity of phospholipids for the GO/CNT surface. Therefore, the rate of lipid hydrolysis was conveniently measured using LDI-TOF mass spectra obtained from a GO/CNT surface on which the phospholipid hydrolysis reaction mixtures had been spotted by comparing the mass-peak intensities of reactants and products. The present platform for phospholipase assays based on MS and GO/CNT double-layer films enables quantitative measurements at low cost, allows assays to be performed in a short time, and is compatible with an array format, unlike conventional assay methods. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | SIGNAL-TRANSDUCTION | - |
dc.subject | CARBON NANOTUBES | - |
dc.subject | PROTEIN-KINASE | - |
dc.subject | HYDROLYSIS | - |
dc.subject | PHOSPHATIDYLCHOLINE | - |
dc.subject | MATRIX | - |
dc.subject | ENZYME | - |
dc.subject | CELLS | - |
dc.subject | METABOLISM | - |
dc.subject | ACTIVATION | - |
dc.title | Laser Desorption/Ionization Mass Spectrometric Assay for Phospholipase Activity Based on Graphene Oxide/Carbon Nanotube Double-Layer Films | - |
dc.type | Article | - |
dc.identifier.wosid | 000283403200009 | - |
dc.identifier.scopusid | 2-s2.0-77958513980 | - |
dc.type.rims | ART | - |
dc.citation.volume | 132 | - |
dc.citation.issue | 42 | - |
dc.citation.beginningpage | 14714 | - |
dc.citation.endingpage | 14717 | - |
dc.citation.publicationname | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | - |
dc.identifier.doi | 10.1021/ja106276j | - |
dc.contributor.localauthor | Min, Dal-Hee | - |
dc.contributor.nonIdAuthor | Lee, Ji-Eon | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | CARBON NANOTUBES | - |
dc.subject.keywordPlus | PROTEIN-KINASE | - |
dc.subject.keywordPlus | HYDROLYSIS | - |
dc.subject.keywordPlus | PHOSPHATIDYLCHOLINE | - |
dc.subject.keywordPlus | MATRIX | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | ACTIVATION | - |
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