DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gwon H.-J. | ko |
dc.contributor.author | Baik S.-H. | ko |
dc.date.accessioned | 2013-03-08T23:37:36Z | - |
dc.date.available | 2013-03-08T23:37:36Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | BIOTECHNOLOGY LETTERS, v.32, no.1, pp.143 - 149 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | http://hdl.handle.net/10203/94677 | - |
dc.description.abstract | Diastereoselectivity-enhanced mutants of l-threonine aldolase (l-TA) for l-threo-3,4-dihydroxyphenylserine (l-threo-DOPS) synthesis were isolated by error-prone PCR followed by a high-throughput screening. The most improved mutant was achieved from the mutant T3-3mm2, showing a 4-fold increase over the wild-type l-TA. When aldol condensation activity was examined using whole cells of T3-3mm2, its de was constantly maintained at 55% during the batch reactions for 80 h, yielding 3.8 mg l-threo-DOPS/ml. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.subject | ALPHA-AMINO ACIDS | - |
dc.subject | GENE CLONING | - |
dc.subject | BIOCATALYSIS | - |
dc.title | Diastereoselective synthesis of l-threo-3,4-dihydroxyphenylserine by low-specific l-threonine aldolase mutants | - |
dc.type | Article | - |
dc.identifier.wosid | 000272565900021 | - |
dc.identifier.scopusid | 2-s2.0-72249087573 | - |
dc.type.rims | ART | - |
dc.citation.volume | 32 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 143 | - |
dc.citation.endingpage | 149 | - |
dc.citation.publicationname | BIOTECHNOLOGY LETTERS | - |
dc.identifier.doi | 10.1007/s10529-009-0125-z | - |
dc.contributor.localauthor | Gwon H.-J. | - |
dc.contributor.nonIdAuthor | Baik S.-H. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Diastereoselectivity | - |
dc.subject.keywordAuthor | L-threo-3,4-Dihydroxyphenylserine | - |
dc.subject.keywordAuthor | L-Threonine aldolase | - |
dc.subject.keywordPlus | ALPHA-AMINO ACIDS | - |
dc.subject.keywordPlus | GENE CLONING | - |
dc.subject.keywordPlus | BIOCATALYSIS | - |
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