Photosensitive polymer brushes grafted onto PTFE film surface for micropatterning of proteins

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Surface grafting of photosensitive polymer brushes on a flexible polymer surface was performed using Ar ion irradiation, which generated peroxides on the polymer surface upon exposure to air. These peroxides were utilized as initiators for graft polymerization of a photosensitive diazoketo-functionalized methacrylate monomer. Upon UV light exposure, the diazoketo group was converted into the carboxylic acid group which was used to covalently immobilize amine-functionalized biotin in a patterned fashion. Successful binding of streptavidin to the biotin-linked regions proved the potential of the platform for biomolecular patterning applications on commercial polymer substrates.
Publisher
ROYAL SOC CHEMISTRY
Issue Date
2010
Language
English
Article Type
Article
Keywords

BIOTIN-BINDING; ACRYLIC-ACID; STREPTAVIDIN; SYSTEM; RESIST; ION; COPOLYMERIZATION; BIOMATERIALS; FABRICATION; MICROARRAYS

Citation

JOURNAL OF MATERIALS CHEMISTRY, v.20, no.10, pp.2007 - 2012

ISSN
0959-9428
DOI
10.1039/b923937e
URI
http://hdl.handle.net/10203/94143
Appears in Collection
CH-Journal Papers(저널논문)
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