DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Tae Jung | ko |
dc.contributor.author | Choi, SK | ko |
dc.contributor.author | Jung, HC | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.contributor.author | Pan, JG | ko |
dc.date.accessioned | 2009-06-15T02:25:24Z | - |
dc.date.available | 2009-06-15T02:25:24Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2009-05 | - |
dc.identifier.citation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.19, no.5, pp.495 - 501 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | http://hdl.handle.net/10203/9396 | - |
dc.description.abstract | A new spore display method is presented that enables recombinant proteins to be displayed on the surface of Bacillus spores via fusion with InhA, an exosporium component of Bacillus thuringiensis. The green fluorescent protein and P-galactosidase as model proteins were fused to the C-terminal region of InhA, respectively. The surface expression of the proteins on the spores was confirmed by flow cytometry, confocal laser scanning microscopy, measurement of the enzyme activity, and an immunogold electron microscopy analysis. InhA-mediated anchoring of foreign proteins in the exosporium of Bacillus spores can provide a new method of microbial display, thereby broadening the potential for novel applications of microbial display. | - |
dc.description.sponsorship | This work was supported by the Korean Systems Biology Research Program (M10309020000-03B5002-00000) of the Korean Ministry of Education, Science, and Technology (T.J.P. and S.Y.L.), the IT Leading R&D Support Project from the Korean Ministry of Knowledge Economy through IITA (T.J.P. and S.Y.L.), and the National Research Laboratory Program of the Korean Ministry of Commerce, Industry, and Energy (S.K.C, H.C.J., and J.G.P.). The authors would also like to thank Hye Jin Choi for her assistance with the TEM imaging (Korea Basic Science Institute, Korea). | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.title | Spore Display Using Bacillus thuringiensis Exosporium Protein InhA | - |
dc.type | Article | - |
dc.identifier.wosid | 000266447800010 | - |
dc.identifier.scopusid | 2-s2.0-67649437199 | - |
dc.type.rims | ART | - |
dc.citation.volume | 19 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 495 | - |
dc.citation.endingpage | 501 | - |
dc.citation.publicationname | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.identifier.doi | 10.4014/jmb.0802.163 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Choi, SK | - |
dc.contributor.nonIdAuthor | Jung, HC | - |
dc.contributor.nonIdAuthor | Pan, JG | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Bacillus thuringiensis | - |
dc.subject.keywordAuthor | spore display | - |
dc.subject.keywordAuthor | exosporium | - |
dc.subject.keywordAuthor | InhA | - |
dc.subject.keywordPlus | BACTERIAL SURFACE DISPLAY | - |
dc.subject.keywordPlus | SUBTILIS SPORES | - |
dc.subject.keywordPlus | CELL-SURFACE | - |
dc.subject.keywordPlus | HETEROLOGOUS PROTEINS | - |
dc.subject.keywordPlus | ANTHRACIS EXOSPORIUM | - |
dc.subject.keywordPlus | METALLOPROTEASE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | LIBRARIES | - |
dc.subject.keywordPlus | ANTIGENS | - |
dc.subject.keywordPlus | CEREUS | - |
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