Carbon sources-dependent carotenoid production in metabolically engineered Escherichia coli

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The effects of three phosphoenolpyruvate (PEP)-dependent PTS carbon sources (glucose, mannose and maltose) and three non-PTS carbon sources (glycerol, galactose, and lactose) on the formation of four carotenoids with diverse structures and on the cell growth of the recombinant Escherichia coli were investigated. The biosynthetic pathways of four carotenoids, C-30 diapolycopene, C-30 diapotorulene, C-40 lycopene, and C-40 beta-carotene, were engineered in E. coli. The resulting E. coli cells were grown in a mineral medium supplemented with each of the six carbon sources. Among the six carbon sources, non-PTS glycerol showed the highest performance in production of all four carotenoid structures, whereas PTS glucose showed the lowest performance. Based on the conversion yield, carotenoid-producing capability, and the cell density, we found that there was no close correlation between PTS and non-PTS transport mechanism and carotenoid formations in E. coli.
Publisher
SPRINGER
Issue Date
2010-12
Language
English
Article Type
Article
Keywords

BACTERIUM METHYLOMONAS SP; PHOSPHOTRANSFERASE SYSTEM; LYCOPENE PRODUCTION; DIRECTED EVOLUTION; E. COLI; BIOSYNTHESIS; EXPRESSION; PATHWAY; COMBINATORIAL; FERMENTATION

Citation

WORLD JOURNAL OF MICROBIOLOGY BIOTECHNOLOGY, v.26, no.12, pp.2231 - 2239

ISSN
0959-3993
URI
http://hdl.handle.net/10203/93534
Appears in Collection
CBE-Journal Papers(저널논문)
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