DC Field | Value | Language |
---|---|---|
dc.contributor.author | Piao, Shunfu | ko |
dc.contributor.author | Song, Young-Lan | ko |
dc.contributor.author | Kim, Jung Hyun | ko |
dc.contributor.author | Park, Sam Yong | ko |
dc.contributor.author | Park, Ji Won | ko |
dc.contributor.author | Lee, Bok Leul | ko |
dc.contributor.author | Oh, Byung-Ha | ko |
dc.contributor.author | Ha, Nam-Chul | ko |
dc.date.accessioned | 2013-03-08T11:14:15Z | - |
dc.date.available | 2013-03-08T11:14:15Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2005-12 | - |
dc.identifier.citation | EMBO JOURNAL, v.24, no.24, pp.4404 - 4414 | - |
dc.identifier.issn | 0261-4189 | - |
dc.identifier.uri | http://hdl.handle.net/10203/92899 | - |
dc.description.abstract | Clip-domain serine proteases (SPs) are the essential components of extracellular signaling cascades in various biological processes, especially in embryonic development and the innate immune responses of invertebrates. They consist of a chymotrypsin-like SP domain and one or two clip domains at the N-terminus. Prophenoloxidase-activating factor (PPAF)-II, which belongs to the noncatalytic clip-domain SP family, is indispensable for the generation of the active phenoloxidase leading to melanization, a major defense mechanism of insects. Here, the crystal structure of PPAF-II reveals that the clip domain adopts a novel fold containing a central cleft, which is distinct from the structures of defensins with a similar arrangement of cysteine residues. Ensuing studies demonstrated that PPAF-II forms a homo-oligomer upon cleavage by the upstream protease and that the clip domain of PPAF-II functions as a module for binding phenoloxidase through the central cleft, while the clip domain of a catalytically active easter-type SP plays an essential role in the rapid activation of its protease domain. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | HOLOTRICHIA-DIOMPHALIA LARVAE | - |
dc.subject | PROPHENOLOXIDASE-ACTIVATING ENZYME | - |
dc.subject | PRO-PHENOL-OXIDASE | - |
dc.subject | DROSOPHILA EMBRYO | - |
dc.subject | PROTEINASE HOMOLOGS | - |
dc.subject | COLEOPTERAN INSECT | - |
dc.subject | MANDUCA-SEXTA | - |
dc.subject | HEMOLYMPH | - |
dc.subject | SYSTEM | - |
dc.subject | ALIGNMENT | - |
dc.title | Crystal structure of a clip-domain serine protease and functional roles of the clip domains | - |
dc.type | Article | - |
dc.identifier.wosid | 000234805100018 | - |
dc.identifier.scopusid | 2-s2.0-29244478349 | - |
dc.type.rims | ART | - |
dc.citation.volume | 24 | - |
dc.citation.issue | 24 | - |
dc.citation.beginningpage | 4404 | - |
dc.citation.endingpage | 4414 | - |
dc.citation.publicationname | EMBO JOURNAL | - |
dc.identifier.doi | 10.1038/sj.emboj.7600891 | - |
dc.contributor.localauthor | Oh, Byung-Ha | - |
dc.contributor.nonIdAuthor | Piao, Shunfu | - |
dc.contributor.nonIdAuthor | Song, Young-Lan | - |
dc.contributor.nonIdAuthor | Kim, Jung Hyun | - |
dc.contributor.nonIdAuthor | Park, Sam Yong | - |
dc.contributor.nonIdAuthor | Park, Ji Won | - |
dc.contributor.nonIdAuthor | Lee, Bok Leul | - |
dc.contributor.nonIdAuthor | Ha, Nam-Chul | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | clip domain | - |
dc.subject.keywordAuthor | easter | - |
dc.subject.keywordAuthor | innate immunity | - |
dc.subject.keywordAuthor | melanin | - |
dc.subject.keywordAuthor | three-dimensional structure | - |
dc.subject.keywordPlus | HOLOTRICHIA-DIOMPHALIA LARVAE | - |
dc.subject.keywordPlus | PROPHENOLOXIDASE-ACTIVATING ENZYME | - |
dc.subject.keywordPlus | PRO-PHENOL-OXIDASE | - |
dc.subject.keywordPlus | DROSOPHILA EMBRYO | - |
dc.subject.keywordPlus | PROTEINASE HOMOLOGS | - |
dc.subject.keywordPlus | COLEOPTERAN INSECT | - |
dc.subject.keywordPlus | MANDUCA-SEXTA | - |
dc.subject.keywordPlus | HEMOLYMPH | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | ALIGNMENT | - |
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