Burkholderia sediminicola sp nov., isolated from freshwater sediment

Abstract

A Gram-negative, motile and rod-shaped bacterium, designated HU2-65W(T), was isolated from freshwater sediment. The strain possessed ubiquinone 8 as the predominant isoprenoid quinone and contained major amounts of omega 7-cis-octadecenoic acid and hexadecanoic acid in its cell envelope, which are properties shared by members of the genus Burkholderia. On the basis of 16S rRNA gene sequence similarity, strain HU2-65WT was most closely related to the type strain of Burkholderia xenovorans (98.4%). The DNA G+C content of strain HU2-65WT was 61.2 mol%, and DNA-DNA relatedness values to type strains of closely related species were found to be much lower than 70%, indicating that the strain represents a separate genomic species within the genus Burkholderia. Strain HU2-65WT was also differentiated from other species of the genus by physiological and biochemical characteristics. Consequently, strain HU2-65WT is considered to represent a single, novel species of the genus Burkholderia, for which the name Burkholderia sediminicola sp. nov. is proposed, with the type strain HU2-65WT (=KCTC 22086(T) =LMG 24238(T)).