Sphingopyxis granuli sp nov., a beta-glucosidase-producing bacterium in the family Sphingomonadaceae in alpha-4 subclass of the Proteobacteria

Abstract

Strain Kw07(T). a Gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from granules in an Up-flow Anaerobic Sludge Blanket (UASB) bioreactor used in the treatment of brewery waste-water. 16S rRNA gene sequence analysis revealed that strain Kw07T belongs to the α-4 subclass of the Proteobacteria, and the highest degree of sequence similarity was determined to be to Sphingopyxis macrogoltabida IFO 15033(T) (97.8%). Chemotaxonomic data revealed that strain Kw07(T) possesses a quinone system with the predominant compound Q-10, the predominant fatty acid C (18:1) ω 7c, and sphingolipids, all of which corroborated our assignment of the strain to the Sphingopyxis genus. The results of DNA-DNA hybridization and physiological and biochemical tests clearly demonstrated that strain Kw07(T) represents a distinct species. Based on these data, Kw07(T) (= KCTC 12209(T) = NBRC 100800(T)) should be classified as the type strain for a novel Sphingopyxis species, for which the name Sphingopyxis granuli sp. nov. has been proposed.