Microlunatus ginsengisoli sp nov, isolated from soil of a ginseng field

Abstract

A Gram-positive, aerobic, coccus-shaped, non-endospore-forming bacterium (Gsoil 633(T)) was isolated from soil from a ginseng field in Pocheon province in South Korea. The novel isolate was characterized in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarities, strain Gsoil 633(T) was shown to belong to the family Propionibacteriaceae. The closest phylogenetic relative was Microlunatus phosphovorus DSM 19555(T), with 96.1 % sequence similarity; the sequence similarity to other members of the family was less than 95.4 %. The isolate was characterized chemotaxonomically as having LL-2,6-diaminopimelic acid in the cell-wall pepticloglycan, MK-9(H-4) as the predominant menaquinone and anteiso-C-15:0, iso-C-15:0 and iSO-C-16:0 as the major fatty acids. The G + C content of the genomic DNA was 69.8 mol%. The morphological and chemotaxonomic properties of the isolate were consistent with those of M. phosphovorus, but the results of physiological and biochemical tests allowed the phenotypic differentiation of strain Gsoil 633(T) from this species. Therefore, strain Gsoil 633(T) represents a novel species, for which the name Microlunatus ginsengisoli sp. nov. is proposed. The type strain is Gsoil 633(T) (= KCTC 13940(T) = DSM 17942(T)).