Chryseobacterium daecheongense sp nov., isolated from freshwater lake sediment

Abstract

A novel nitrate-reducing bacterium, CPW406(T), was isolated from the sediment of a shallow, freshwater lake. The strain was a Gram-negative, non-motile, non-spore-forming rod, which formed yellow-pigmented colonies on nutrient agar and contained a polyamine pattern with sym-homospermidine as the major compound, MK-6 as the predominant menaquinone, 15: 0 iso and 17 : 0 iso, 3-OH as the major fatty acids and phosphatidylethanolamine and several unknown lipids in the polar lipid profile. The 16S rRNA gene sequence of strain CPW406(T) was found to be most similar to that of the type strain of Chryseobacterium defluvii (DSM 14219(T); 97(.)9 % similarity). However, DNA-DNA relatedness data and its phenotypic properties showed that strain CPW406(T) could be distinguished from all known Chryseobacterium soecies and thus represented a novel species, for which the name Chryseobacterium daecheongense sp. nov. is proposed; the type strain is CPW406(T) (=DSM 15235(T) =KCTC 12088(T)).