DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bae, SW | ko |
dc.contributor.author | Lee, Gyun-Min | ko |
dc.date.accessioned | 2013-03-08T08:23:41Z | - |
dc.date.available | 2013-03-08T08:23:41Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2005-06 | - |
dc.identifier.citation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.15, pp.579 - 586 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | http://hdl.handle.net/10203/92590 | - |
dc.description.abstract | When subjected to hyperosmotic pressure by NaCl addition, H69K-NGD transfectoma, like KR12H-2 transfectoma, displayed decreased specific growth rate (p) and increased specific antibody productivity (q(Ab)): Elevation of medium osmolality from 280 mOsm/kg to 415 mOsm/kg decreased P by 79% in batch cultures of H69K-NGD transfectoma, while it increased q(Ab) 103%. However, unlike KR12H-2 tranfectoma, enhanced q(Ab) of H69K-NGD transfectoma at hyperosmolalities was not due to elevated levels of Ig mRNAs. In hyperosmotic cultures of H69K-NGD transfectoma, heavy-chain mRNA per cell was not enhanced with increasing osmolality. Hyperosmotic pressure was found to preferentially enhance immunoglobulin (Ig) translation rates of H69K-NGD transfectoma. However, under hyperosmotic pressure, the translation rate of Ig polypeptides was not enhanced as much as q(Ab). This result suggests that hyperosmotic pressure also influences the post-translational process. Taken together, the results obtained show that intracellular response of transfectomas to hyperosmotic pressure, in regard to the main intracellular steps of the antibody secretory pathway, is cell-fine dependent. | - |
dc.language | English | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | HAMSTER OVARY CELLS | - |
dc.subject | HEPATITIS-B VIRUS | - |
dc.subject | SODIUM-BUTYRATE | - |
dc.subject | MONOCLONAL-ANTIBODIES | - |
dc.subject | OSMOTIC-PRESSURE | - |
dc.subject | CHO-CELLS | - |
dc.subject | CLONING | - |
dc.subject | EXPRESSION | - |
dc.subject | PROTEINS | - |
dc.subject | CULTURE | - |
dc.title | Intracellular responses of antibody-producing H69K-NGD transfectoma subjected to hyperosmotic pressure | - |
dc.type | Article | - |
dc.identifier.wosid | 000230149300020 | - |
dc.identifier.scopusid | 2-s2.0-22544475901 | - |
dc.type.rims | ART | - |
dc.citation.volume | 15 | - |
dc.citation.beginningpage | 579 | - |
dc.citation.endingpage | 586 | - |
dc.citation.publicationname | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.contributor.localauthor | Lee, Gyun-Min | - |
dc.contributor.nonIdAuthor | Bae, SW | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | antibody | - |
dc.subject.keywordAuthor | hyperosmotic pressure | - |
dc.subject.keywordAuthor | transfectoma | - |
dc.subject.keywordPlus | HAMSTER OVARY CELLS | - |
dc.subject.keywordPlus | HEPATITIS-B VIRUS | - |
dc.subject.keywordPlus | SODIUM-BUTYRATE | - |
dc.subject.keywordPlus | MONOCLONAL-ANTIBODIES | - |
dc.subject.keywordPlus | OSMOTIC-PRESSURE | - |
dc.subject.keywordPlus | CHO-CELLS | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | CULTURE | - |
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