DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sung, Yun-Hee | ko |
dc.contributor.author | Lee, Jae-Seong | ko |
dc.contributor.author | Park, Soon-Hye | ko |
dc.contributor.author | Koo, Jane | ko |
dc.contributor.author | Lee, Gyun-Min | ko |
dc.date.accessioned | 2013-03-08T08:11:53Z | - |
dc.date.available | 2013-03-08T08:11:53Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007-09 | - |
dc.identifier.citation | METABOLIC ENGINEERING, v.9, pp.452 - 464 | - |
dc.identifier.issn | 1096-7176 | - |
dc.identifier.uri | http://hdl.handle.net/10203/92568 | - |
dc.description.abstract | Previously, the expression of caspase-3 siRNA could not effectively inhibit sodium butyrate (NaBu)-induced apoptotic cell death of recombinant Chinese hamster ovary (rCHO) cells producing human thrombopoietin (hTPO). Caspase-3 siRNA expressing cells appeared to compensate for the lack of caspase-3 by increasing active caspase-7 levels. For the successful inhibition of NaBu-induced apoptosis of rCHO cells, both caspase-3 and caspase-7 were down-regulated using the siRNA expression vector system. Co-down-regulation of caspase-3 and caspase-7 increased cell viability and extended culture longevity in serum-free culture in the presence or absence of 1mM NaBu addition. In the cultures with 1mM NaBu addition, the maximum hTPO concentration in rCHO cells with down-regulation of both caspases was approximately 55% higher than that in rCHO cells without down-regulation of caspases and approximately 16% higher than rCHO cells with down-regulation of only caspase-3. However, in the culture with 3mM NaBu, this strategy could not dramatically enhance the culture longevity and hTPO production, compared to Bcl-2 overexpression. The different result in hTPO production between down-regulation of caspases and Bcl-2 overexpression may be because the down-regulation of caspase-3 and caspase-7, unlike Bcl-2 overexpression, could not maintain mitochondrial membrane potential in the presence of 3mM NaBu. Taken together, co-down-regulation of caspase-3 and caspase-7 is effective in regard to extension of culture longevity and enhancement of hTPO production in a serum-free culture in the presence or absence of 1mM NaBu addition. (C) 2007 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | SHORT INTERFERING RNAS | - |
dc.subject | MAMMALIAN-CELLS | - |
dc.subject | CHO-CELLS | - |
dc.subject | MEGAKARYOCYTE GROWTH | - |
dc.subject | ANTIBODY-PRODUCTION | - |
dc.subject | ENHANCED PRODUCTION | - |
dc.subject | MESSENGER-RNA | - |
dc.subject | CULTURE | - |
dc.subject | INHIBITION | - |
dc.subject | EXPRESSION | - |
dc.title | Influence of co-down-regulation of caspase-3 and caspase-7 by siRNAs on sodium butyrate-induced apoptotic cell death of Chinese hamster ovary cells producing thrombopoietin | - |
dc.type | Article | - |
dc.identifier.wosid | 000253485400006 | - |
dc.identifier.scopusid | 2-s2.0-35549010214 | - |
dc.type.rims | ART | - |
dc.citation.volume | 9 | - |
dc.citation.beginningpage | 452 | - |
dc.citation.endingpage | 464 | - |
dc.citation.publicationname | METABOLIC ENGINEERING | - |
dc.identifier.doi | 10.1016/j.ymben.2007.08.001 | - |
dc.contributor.localauthor | Lee, Gyun-Min | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | caspase-3 | - |
dc.subject.keywordAuthor | caspase-7 | - |
dc.subject.keywordAuthor | sodium butyrate | - |
dc.subject.keywordAuthor | Chinese hamster ovary (CHO) cells | - |
dc.subject.keywordAuthor | small interfering RNA (siRNA) | - |
dc.subject.keywordPlus | SHORT INTERFERING RNAS | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | CHO-CELLS | - |
dc.subject.keywordPlus | MEGAKARYOCYTE GROWTH | - |
dc.subject.keywordPlus | ANTIBODY-PRODUCTION | - |
dc.subject.keywordPlus | ENHANCED PRODUCTION | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | EXPRESSION | - |
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