DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hwang, Su-Jeong | ko |
dc.contributor.author | Kim, Sung-Hyun | ko |
dc.contributor.author | Kim, Hak-Zoo | ko |
dc.contributor.author | Steinmetz, Michel O | ko |
dc.contributor.author | Koh, Gou-Young | ko |
dc.contributor.author | Lee, Gyun-Min | ko |
dc.date.accessioned | 2013-03-08T08:11:20Z | - |
dc.date.available | 2013-03-08T08:11:20Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2008-08 | - |
dc.identifier.citation | PROTEIN JOURNAL, v.27, pp.319 - 326 | - |
dc.identifier.issn | 1572-3887 | - |
dc.identifier.uri | http://hdl.handle.net/10203/92567 | - |
dc.description.abstract | A designed angiopoietin-1 (Ang1) chimeric protein with nonleaky angiogenic activity, COMP-Ang1, is an effective alternative to native Ang1 for therapeutic angiogenesis in vivo. Recombinant Chinese hamster ovary (rCHO) cell lines expressing a high level (> 20 mu g/mL) of COMP-Ang1 and an amino-terminal FLAG-tag were constructed by transfecting the expression vector into dihydrofolate reductase-deficient CHO cells and the subsequent gene amplification in medium containing stepwise increments in methotrexate level such as 0.02, 0.08, 0.32, and 1 mu M. The COMP-Ang1 secreted from rCHO cells was purified at a purification yield of 40.3% from the culture medium using an anti-FLAG M2 agarose affinity gel. SDS-PAGE and Western blot analyses showed that rCHO cells secrete COMP-Ang1 in homopentameric and homotetrameric glycoprotein forms. Furthermore, COMP-Ang1 binds to the Tie2 receptor and phosphorylates Tie2, indicating its potential for therapeutic angiogenesis. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.subject | MAMMALIAN-CELLS | - |
dc.subject | BLOOD-FLOW | - |
dc.subject | CHO CELLS | - |
dc.subject | RECOMBINANT | - |
dc.subject | COMP-ANGIOPOIETIN-1 | - |
dc.subject | SECRETION | - |
dc.subject | APOPTOSIS | - |
dc.subject | RECEPTOR | - |
dc.subject | VARIANT | - |
dc.subject | LIGAND | - |
dc.title | High-level expression and purification of a designed angiopoietin-1 chimeric protein, COMP-Ang1, produced in Chinese hamster ovary cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000259009400007 | - |
dc.identifier.scopusid | 2-s2.0-51449106720 | - |
dc.type.rims | ART | - |
dc.citation.volume | 27 | - |
dc.citation.beginningpage | 319 | - |
dc.citation.endingpage | 326 | - |
dc.citation.publicationname | PROTEIN JOURNAL | - |
dc.identifier.doi | 10.1007/s10930-008-9140-5 | - |
dc.contributor.localauthor | Koh, Gou-Young | - |
dc.contributor.localauthor | Lee, Gyun-Min | - |
dc.contributor.nonIdAuthor | Kim, Sung-Hyun | - |
dc.contributor.nonIdAuthor | Kim, Hak-Zoo | - |
dc.contributor.nonIdAuthor | Steinmetz, Michel O | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | angiopoietin | - |
dc.subject.keywordAuthor | COMP-Ang1 | - |
dc.subject.keywordAuthor | FLAG-tag | - |
dc.subject.keywordAuthor | Tie2 receptor | - |
dc.subject.keywordAuthor | Chinese hamster ovary cells | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | BLOOD-FLOW | - |
dc.subject.keywordPlus | CHO CELLS | - |
dc.subject.keywordPlus | RECOMBINANT | - |
dc.subject.keywordPlus | COMP-ANGIOPOIETIN-1 | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | VARIANT | - |
dc.subject.keywordPlus | LIGAND | - |
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