Investigation of the doughnut effect in Cy3-labeled protein microarrays using scanning near-field optical microscope

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We demonstrate the fluorescence mapping of protein microarrays by the technique of scanning near-field optical microscopy (SNOM) and confocal microscopy. Micron sized spots (300 mu m) of human Immunoglobutin G (hIgG) protein with and without a Cy3 dye labeling have been fabricated on glass substrates by an immobilization method which makes use of calixcrown derivatives termed Prolinker. We have also tried to probe into the well-known "doughnut effect" observed in fluorescence images of proteins using the SNOM technique. The topographic and fluorescence SNOM images revealed that the number of proteins at the boundary of the spot were more than at the center in the case of the microarray spot which showed brighter luminescence at the edge than at the center in the confocal image. (c) 2006 The Optical Society of Japan.
Publisher
OPTICAL SOC JAPAN
Issue Date
2006-07
Language
English
Article Type
Article; Proceedings Paper
Citation

OPTICAL REVIEW, v.13, pp.288 - 291

ISSN
1340-6000
DOI
10.1007/s10043-006-0288-y
URI
http://hdl.handle.net/10203/91591
Appears in Collection
PH-Journal Papers(저널논문)
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