DC Field | Value | Language |
---|---|---|
dc.contributor.author | NAM, YOONKEY | ko |
dc.contributor.author | Brewer, GJ | ko |
dc.contributor.author | Wheeler, BC | ko |
dc.date.accessioned | 2013-03-07T19:50:48Z | - |
dc.date.available | 2013-03-07T19:50:48Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007-08 | - |
dc.identifier.citation | JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, v.18, pp.1091 - 1100 | - |
dc.identifier.issn | 0920-5063 | - |
dc.identifier.uri | http://hdl.handle.net/10203/91141 | - |
dc.description.abstract | The purpose of this work was to study the development of astroglial cells in patterned neuronal cultures. Hippocampal neurons, derived from embryonic stage (E18) rats and cultured in serum-free Neurobasal/B27 medium, grew to follow patterns of poly(D-lysine) created by microcontact printing. The growth of the astroglial cells and the co-localization of neurons and astroglial cells were measured for up to one month using fluorescence immunostaining of neurons and astroglial cells. Neurons grew to form square patterns within 2 weeks, while astroglia only started to emerge in the same period. Astroglial cells continued to proliferate for a month following a general growth curve. Over 90% of the astroglial cell area co-localized with neurons (within 2 mu m) at an early stage of astroglial development (13 DIV). Over the remaining period, astroglial cells proliferated and the co-localization was 80%. Hence, in these culture conditions astroglial cells develop 2-3 weeks later than neurons but remain highly co-localized with neurons. | - |
dc.language | English | - |
dc.publisher | VSP BV | - |
dc.title | Development of astroglial cells in patterned neuronal cultures | - |
dc.type | Article | - |
dc.identifier.wosid | 000249005300010 | - |
dc.identifier.scopusid | 2-s2.0-34548044244 | - |
dc.type.rims | ART | - |
dc.citation.volume | 18 | - |
dc.citation.beginningpage | 1091 | - |
dc.citation.endingpage | 1100 | - |
dc.citation.publicationname | JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION | - |
dc.identifier.doi | 10.1163/156856207781494430 | - |
dc.contributor.localauthor | NAM, YOONKEY | - |
dc.contributor.nonIdAuthor | Brewer, GJ | - |
dc.contributor.nonIdAuthor | Wheeler, BC | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | patterned neuronal network in vitro | - |
dc.subject.keywordAuthor | hippocampal neurons | - |
dc.subject.keywordAuthor | astroglial cells | - |
dc.subject.keywordAuthor | serum-free culture medium | - |
dc.subject.keywordPlus | HIPPOCAMPAL-NEURONS | - |
dc.subject.keywordPlus | NETWORKS | - |
dc.subject.keywordPlus | SURFACES | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | POLYLYSINE | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.subject.keywordPlus | POLARITY | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | INVITRO | - |
dc.subject.keywordPlus | GLIA | - |
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