Beyond toothpicks: new methods for isolating mutant bacteria

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Over the past 50 years genetic analysis in microbiology has relied predominantly on selections and plate assays using chromogenic enzyme substrates-for example, X-gal assays for the detection of beta-galactosidase activity. Recent advances in fluorescent assays and high throughput screening technologies have paved the way for the rapid isolation of mutants that confer complex phenotypes and for the quantitative analysis of the evolution of new traits in bacterial populations. This Review highlights the power of novel single-cell screening technologies and their applications to genetics, evolution and the biotechnological uses of bacteria.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2007-09
Language
English
Article Type
Review
Keywords

GREEN FLUORESCENT PROTEIN; ACTIVATED CELL SORTER; ESCHERICHIA-COLI; DIRECTED EVOLUTION; SURFACE-DISPLAY; FLOW-CYTOMETRY; PERIPLASMIC EXPRESSION; PHYSIOLOGICAL-STATE; MOLECULAR EVOLUTION; ANTIBODY FRAGMENTS

Citation

NATURE REVIEWS MICROBIOLOGY, v.5, no.9, pp.680 - 688

ISSN
1740-1526
DOI
10.1038/nrmicro1715
URI
http://hdl.handle.net/10203/90610
Appears in Collection
CBE-Journal Papers(저널논문)
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