DC Field | Value | Language |
---|---|---|
dc.contributor.author | Galvez, Thierry | ko |
dc.contributor.author | Teruel, Mary N. | ko |
dc.contributor.author | Heo, Won Do | ko |
dc.contributor.author | Jones, Joshua T. | ko |
dc.contributor.author | Kim, Man Lyang | ko |
dc.contributor.author | Liou, Jen | ko |
dc.contributor.author | Myers, Jason W. | ko |
dc.contributor.author | Meyer, Tobias | ko |
dc.date.accessioned | 2013-03-07T14:48:10Z | - |
dc.date.available | 2013-03-07T14:48:10Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | GENOME BIOLOGY, v.8, no.7 | - |
dc.identifier.issn | 1474-7596 | - |
dc.identifier.uri | http://hdl.handle.net/10203/90427 | - |
dc.description.abstract | Background: Iron uptake via endocytosis of iron-transferrin-transferrin receptor complexes is a rate-limiting step for cell growth, viability and proliferation in tumor cells as well as non-transformed cells such as activated lymphocytes. Signaling pathways that regulate transferrin uptake have not yet been identified. Results: We surveyed the human signaling proteome for regulators that increase or decrease transferrin uptake by screening 1,804 dicer-generated signaling small interfering RNAs using automated quantitative imaging. In addition to known transport proteins, we identified 11 signaling proteins that included a striking signature set for the phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5) P3)-target of rapamycin (mTOR) signaling pathway. We show that the PI3K- mTOR signaling pathway is a positive regulator of transferrin uptake that increases the number of transferrin receptors per endocytic vesicle without affecting endocytosis or recycling rates. Conclusion: Our study identifies the PtdIns( 3,4,5) P3-mTOR signaling pathway as a new regulator of iron-transferrin uptake and serves as a proof-of-concept that targeted RNA interference screens of the signaling proteome provide a powerful and unbiased approach to discover or rank signaling pathways that regulate a particular cell function. | - |
dc.language | English | - |
dc.publisher | BIOMED CENTRAL LTD | - |
dc.title | siRNA screen of the human signaling proteome identifies the PtdIns(3,4,5) P-3-mTOR signaling pathway as a primary regulator of transferrin uptake | - |
dc.type | Article | - |
dc.identifier.wosid | 000249416400022 | - |
dc.identifier.scopusid | 2-s2.0-39749108111 | - |
dc.type.rims | ART | - |
dc.citation.volume | 8 | - |
dc.citation.issue | 7 | - |
dc.citation.publicationname | GENOME BIOLOGY | - |
dc.identifier.doi | 10.1186/gb-2007-8-7-r142 | - |
dc.contributor.localauthor | Heo, Won Do | - |
dc.contributor.nonIdAuthor | Galvez, Thierry | - |
dc.contributor.nonIdAuthor | Teruel, Mary N. | - |
dc.contributor.nonIdAuthor | Jones, Joshua T. | - |
dc.contributor.nonIdAuthor | Kim, Man Lyang | - |
dc.contributor.nonIdAuthor | Liou, Jen | - |
dc.contributor.nonIdAuthor | Myers, Jason W. | - |
dc.contributor.nonIdAuthor | Meyer, Tobias | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | MAMMALIAN TARGET | - |
dc.subject.keywordPlus | MEDIATED ENDOCYTOSIS | - |
dc.subject.keywordPlus | RECEPTOR GENE | - |
dc.subject.keywordPlus | CELL | - |
dc.subject.keywordPlus | MTOR | - |
dc.subject.keywordPlus | IRON | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | RAPAMYCIN | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | GROWTH | - |
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