SOMNUS, a CCCH-type zinc finger protein in Arabidopsis, negatively regulates light-dependent seed germination downstream of PIL5

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Light absorbed by seed phytochromes of Arabidopsis thaliana modulates abscisic acid (ABA) and gibberellic acid (GA) signaling pathways at least partly via PHYTOCHROME-INTERACTING FACTOR3-LIKE5 (PIL5), a phytochrome-interacting basic helix-loop-helix transcription factor. Here, we report a new mutant, somnus (som), that germinates in darkness, independently of various light regimens. SOM encodes a nucleus-localized CCCH-type zinc finger protein. The som mutant has lower levels of ABA and elevated levels of GA due to expressional changes in ABA and GA metabolic genes. Unlike PIL5, however, SOM does not regulate the expression of GA-INSENSITIVE and REPRESSOR OF GA1 (RGA/RGA1), two DELLA genes encoding GA negative signaling components. Our in vivo analysis shows that PIL5 activates the expression of SOM by binding directly to its promoter, suggesting that PIL5 regulates ABA and GA metabolic genes partly through SOM. In agreement with these results, we also observed that the reduced germination frequency of a PIL5 overexpression line is rescued by the som mutation and that this rescue is accompanied by expressional changes in ABA and GA metabolic genes. Taken together, our results indicate that SOM is a component in the phytochrome signal transduction pathway that regulates hormone metabolic genes downstream of PIL5 during seed germination.
Publisher
AMER SOC PLANT BIOLOGISTS
Issue Date
2008-05
Language
English
Article Type
Article
Keywords

THALIANA L HEYNH; PHYTOCHROME INTERACTING FACTOR-3; PLANT PHOTORECEPTORS PHYTOCHROME; AU-RICH ELEMENTS; ABSCISIC-ACID; TRANSCRIPTION FACTOR; MESSENGER-RNA; GIBBERELLIN BIOSYNTHESIS; FUNCTIONAL-ANALYSIS; SIGNALING NETWORKS

Citation

PLANT CELL, v.20, no.5, pp.1260 - 1277

ISSN
1040-4651
DOI
10.1105/tpc.108.058859
URI
http://hdl.handle.net/10203/90375
Appears in Collection
BS-Journal Papers(저널논문)
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