DC Field | Value | Language |
---|---|---|
dc.contributor.author | Connell, Laureen E. | ko |
dc.contributor.author | Helfman, David M | ko |
dc.date.accessioned | 2013-03-07T14:04:52Z | - |
dc.date.available | 2013-03-07T14:04:52Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2006-06 | - |
dc.identifier.citation | JOURNAL OF CELL SCIENCE, v.119, no.11, pp.2269 - 2281 | - |
dc.identifier.issn | 0021-9533 | - |
dc.identifier.uri | http://hdl.handle.net/10203/90354 | - |
dc.description.abstract | Myosin II activation is essential for stress fiber and focal adhesion formation, and is implicated in integrin-mediated signaling events. In this study we investigated the role of acto-myosin contractility, and its main regulators, i. e. myosin light chain kinase (MLCK) and Rho-kinase ( ROCK) in cell survival in normal and Ras- transformed MCF-10A epithelial cells. Treatment of cells with pharmacological inhibitors of MLCK ( ML-7 and ML-9), or expression of dominant- negative MLCK, led to apoptosis in normal and transformed MCF- 10A cells. By contrast, treatment of cells with a ROCK inhibitor ( Y- 27632) did not induce apoptosis in these cells. Apoptosis following inhibition of myosin II activation by MLCK is probably meditated through the death receptor pathway because expression of dominant- negative FADD blocked apoptosis. The apoptosis observed after MLCK inhibition is rescued by pre- treatment of cells with integrin- activating antibodies. In addition, this rescue of apoptosis is dependent on FAK activity, suggesting the participation of an integrin- dependent signaling pathway. These studies demonstrate a newly discovered role for MLCK in the generation of pro- survival signals in both untransformed and transformed epithelial cells and supports previous work suggesting distinct cellular roles for Rho- kinase- and MLCK- dependent regulation of myosin II. | - |
dc.language | English | - |
dc.publisher | COMPANY OF BIOLOGISTS LTD | - |
dc.subject | FOCAL ADHESION KINASE | - |
dc.subject | ACTIN CYTOSKELETON | - |
dc.subject | MAMMARY TUMORIGENESIS | - |
dc.subject | EXTRACELLULAR-MATRIX | - |
dc.subject | MLC PHOSPHORYLATION | - |
dc.subject | CATALYTIC-ACTIVITY | - |
dc.subject | DISTINCT ROLES | - |
dc.subject | STRESS FIBERS | - |
dc.subject | DAP-KINASE | - |
dc.subject | RHO-KINASE | - |
dc.title | Myosin light chain kinase plays a role in the regulation of epithelial cell survival | - |
dc.type | Article | - |
dc.identifier.wosid | 000238413300010 | - |
dc.identifier.scopusid | 2-s2.0-33745505877 | - |
dc.type.rims | ART | - |
dc.citation.volume | 119 | - |
dc.citation.issue | 11 | - |
dc.citation.beginningpage | 2269 | - |
dc.citation.endingpage | 2281 | - |
dc.citation.publicationname | JOURNAL OF CELL SCIENCE | - |
dc.identifier.doi | 10.1242/jcs.02926 | - |
dc.contributor.localauthor | Helfman, David M | - |
dc.contributor.nonIdAuthor | Connell, Laureen E. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | acto-myosin contractility | - |
dc.subject.keywordAuthor | MLCK | - |
dc.subject.keywordAuthor | ROCK | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordPlus | FOCAL ADHESION KINASE | - |
dc.subject.keywordPlus | ACTIN CYTOSKELETON | - |
dc.subject.keywordPlus | MAMMARY TUMORIGENESIS | - |
dc.subject.keywordPlus | EXTRACELLULAR-MATRIX | - |
dc.subject.keywordPlus | MLC PHOSPHORYLATION | - |
dc.subject.keywordPlus | CATALYTIC-ACTIVITY | - |
dc.subject.keywordPlus | DISTINCT ROLES | - |
dc.subject.keywordPlus | STRESS FIBERS | - |
dc.subject.keywordPlus | DAP-KINASE | - |
dc.subject.keywordPlus | RHO-KINASE | - |
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