Dielectrophoretic oocyte selection chip for in vitro fertilization

Cited 54 time in webofscience Cited 0 time in scopus
  • Hit : 382
  • Download : 0
This paper reports a new dielectrophoretic separation method of porcine oocytes for in vitro fertilization. Conventional manual selection of oocyte highly depends on the expert's experience and lacks universal standards for identifying the quality of oocyte. In this study, an electrode array chip with castellated shape was developed to evaluate dielectrophoretic velocities of oocytes, under applied bias conditions with an AC 3 V waveform at 1 MHz for 15 s. Based on different dielectrophoresis (DEP) response, the selected group of oocytes that moved showed a better developmental potential than the group of oocytes that stayed, representing a higher rate of blastocyst formation and a lower rate of polyspermic fertilization. In addition, the overall developmental potential of oocytes selected by the DEP device was comparable to that of oocytes selected by conventional manual method. These results demonstrate that the difference in dielectrophoretic velocity can be used to establish an objective criterion for the selection of oocytes. Consequently, this method will open the possibility to develop an automatic tool for oocyte selection, which would be helpful for assisted reproductive technologies such as transgenic and clonal animal production.
Publisher
SPRINGER
Issue Date
2008-06
Language
English
Article Type
Article
Keywords

ON-A-CHIP; MICROFLUIDIC SYSTEM; CELLS; SEPARATION; EMBRYOS; MICROSYSTEMS; MEMBRANE; CULTURE; QUALITY; DEVICE

Citation

BIOMEDICAL MICRODEVICES, v.10, no.3, pp.337 - 345

ISSN
1387-2176
DOI
10.1007/s10544-007-9141-8
URI
http://hdl.handle.net/10203/90256
Appears in Collection
BiS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 54 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0