Diffraction phase and fluorescence microscopy

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We have developed diffraction phase and fluorescence (DPF) microscopy as a new technique for simultaneous quantitative phase imaging and epi-fluorescence investigation of live cells. The DPF instrument consists of an interference microscope, which is incorporated into a conventional inverted fluorescence microscope. The quantitative phase images are characterized by sub-nanometer optical path-length stability over periods from milliseconds to a cell lifetime. The potential of the technique for quantifying rapid nanoscale motions in live cells is demonstrated by experiments on red blood cells, while the composite phase-fluorescence imaging mode is exemplified with mitotic kidney cells. (c) 2006 Optical Society of America
Publisher
OPTICAL SOC AMER
Issue Date
2006-09
Language
English
Article Type
Article
Keywords

DYNAMICS; CELLS; INTERFEROMETER; SUBWAVELENGTH; LIGHT

Citation

OPTICS EXPRESS, v.14, no.18, pp.8263 - 8268

ISSN
1094-4087
DOI
10.1364/OE.14.008263
URI
http://hdl.handle.net/10203/90113
Appears in Collection
PH-Journal Papers(저널논문)
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