DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jungwoon | ko |
dc.contributor.author | Kim, Hye Kyoung | ko |
dc.contributor.author | Rho, Jeung-Yon | ko |
dc.contributor.author | Han, Yong Mahn | ko |
dc.contributor.author | Kim, Jungho | ko |
dc.date.accessioned | 2013-03-07T10:27:48Z | - |
dc.date.available | 2013-03-07T10:27:48Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2006-11 | - |
dc.identifier.citation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.281, no.44, pp.33554 - 33565 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/10203/89967 | - |
dc.description.abstract | OCT-4 transcription factors play an important role in maintaining the pluripotent state of embryonic stem cells and may prevent expression of genes activated during differentiation. Human OCT-4 isoformm RNAs encode proteins that have identical POU DNA binding domains and C-terminal domains but differ in their N-terminal domains. We report here the cloning and characterization of the human OCT-4B isoform. Human OCT-4B cDNA encodes a 265-amino acid protein with a predicted molecular mass of 30 kDa. Embryonic stem (ES) cell-based complementation assays using ZHBTc4 ES cells showed that unlike human OCT-4A, OCT-4B cannot sustain ES cell self-renewal. In addition, OCT-4B does not bind to a probe carrying the OCT-4 consensus binding sequence, and we demonstrate that two separate regions of its N-terminal domain are responsible for inhibiting DNA binding. We also demonstrate that OCT-4B is mainly localized to the cytoplasm. Overexpression of OCT-4B did not activate transcription from OCT-4-dependent promoters, although OCT-4A did as reported previously. Furthermore, transcriptional activation by human OCT-4A was not inhibited by co-expression of OCT-4B. Taken together, these data suggest that the DNA binding, transactivation, and abilities to confer self-renewal of the human OCT-4 isoforms differ. | - |
dc.language | English | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.subject | EMBRYONIC STEM-CELLS | - |
dc.subject | HUMAN CHORIONIC-GONADOTROPIN | - |
dc.subject | TRANSCRIPTION FACTOR OCT-3/4 | - |
dc.subject | POU-DOMAIN | - |
dc.subject | CHROMOSOMAL LOCATION | - |
dc.subject | MOUSE EMBRYO | - |
dc.subject | GERM-LINE | - |
dc.subject | MAMMALIAN EMBRYO | - |
dc.subject | ES CELLS | - |
dc.subject | GENE | - |
dc.title | The human OCT-4 isoforms differ in their ability to confer self-renewal | - |
dc.type | Article | - |
dc.identifier.wosid | 000241621400065 | - |
dc.identifier.scopusid | 2-s2.0-33845930820 | - |
dc.type.rims | ART | - |
dc.citation.volume | 281 | - |
dc.citation.issue | 44 | - |
dc.citation.beginningpage | 33554 | - |
dc.citation.endingpage | 33565 | - |
dc.citation.publicationname | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.identifier.doi | 10.1074/jbc.M603937200 | - |
dc.contributor.localauthor | Han, Yong Mahn | - |
dc.contributor.nonIdAuthor | Lee, Jungwoon | - |
dc.contributor.nonIdAuthor | Kim, Hye Kyoung | - |
dc.contributor.nonIdAuthor | Rho, Jeung-Yon | - |
dc.contributor.nonIdAuthor | Kim, Jungho | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | EMBRYONIC STEM-CELLS | - |
dc.subject.keywordPlus | HUMAN CHORIONIC-GONADOTROPIN | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR OCT-3/4 | - |
dc.subject.keywordPlus | POU-DOMAIN | - |
dc.subject.keywordPlus | CHROMOSOMAL LOCATION | - |
dc.subject.keywordPlus | MOUSE EMBRYO | - |
dc.subject.keywordPlus | GERM-LINE | - |
dc.subject.keywordPlus | MAMMALIAN EMBRYO | - |
dc.subject.keywordPlus | ES CELLS | - |
dc.subject.keywordPlus | GENE | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.