DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ryu, Kyoung-Seok | ko |
dc.contributor.author | Choi, Yun-Seok | ko |
dc.contributor.author | Ko, Jun-Sang | ko |
dc.contributor.author | Kim, Seong-Ock | ko |
dc.contributor.author | Kim, Hyun-Jung | ko |
dc.contributor.author | Cheong, Hae-Kap | ko |
dc.contributor.author | Jeon, Young-Ho | ko |
dc.contributor.author | Choi, Byong-Seok | ko |
dc.contributor.author | Cheong, Chae-Joon | ko |
dc.date.accessioned | 2013-03-07T10:22:00Z | - |
dc.date.available | 2013-03-07T10:22:00Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2008-12 | - |
dc.identifier.citation | BMB REPORTS, v.41, no.12, pp.852 - 857 | - |
dc.identifier.issn | 1976-6696 | - |
dc.identifier.uri | http://hdl.handle.net/10203/89961 | - |
dc.description.abstract | Little attention has been paid to the specificity between E2 and the target protein during ubiquitination, although RING-E3 induces a potential intra-molecular reaction by mediating the direct transfer of ubiquitin from E2 to the target protein. We have constructed artificial E2 fusion proteins in which a target protein (p27) is tethered to one of six E2s via a flexible linker. Interestingly, only three E2s (UbcH5b, hHR6b, and Cdc34) are able to ubiquitinate p27 via an intra-molecular reaction in this system. Although the first ubiquitination of p27 (p27-Ub) by Cdc34 is less efficient than that of UbcH5b and hHR6b, the additional ubiquitin attachment to p27-Ub by Cdc34 is highly efficient. The E2 core of Cdc34 provides specificity to p27, and the residues 184-196 are required for possessive ubiquitination by Cdc34. We demonstrate direct E2 specificity for p27 and also show that differential ubiquitin linkages can be dependent on E2 alone. [BMB reports 2008; 41(12): 852-857] | - |
dc.language | English | - |
dc.publisher | KOREAN SOCIETY BIOCHEMISTRY MOLECULAR BIOLOGY | - |
dc.subject | LIGASE COMPLEX | - |
dc.subject | ENZYME CDC34 | - |
dc.subject | CHAIN | - |
dc.subject | RECOGNITION | - |
dc.subject | P27(KIP1) | - |
dc.subject | DEGRADATION | - |
dc.subject | MECHANISMS | - |
dc.subject | BINDING | - |
dc.subject | REPAIR | - |
dc.subject | SUMO | - |
dc.title | Direct characterization of E2-dependent target specificity and processivity using an artificial p27-linker-E2 ubiquitination system | - |
dc.type | Article | - |
dc.identifier.wosid | 000262297600005 | - |
dc.identifier.scopusid | 2-s2.0-58149348710 | - |
dc.type.rims | ART | - |
dc.citation.volume | 41 | - |
dc.citation.issue | 12 | - |
dc.citation.beginningpage | 852 | - |
dc.citation.endingpage | 857 | - |
dc.citation.publicationname | BMB REPORTS | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Choi, Byong-Seok | - |
dc.contributor.nonIdAuthor | Ryu, Kyoung-Seok | - |
dc.contributor.nonIdAuthor | Choi, Yun-Seok | - |
dc.contributor.nonIdAuthor | Ko, Jun-Sang | - |
dc.contributor.nonIdAuthor | Kim, Hyun-Jung | - |
dc.contributor.nonIdAuthor | Cheong, Hae-Kap | - |
dc.contributor.nonIdAuthor | Jeon, Young-Ho | - |
dc.contributor.nonIdAuthor | Cheong, Chae-Joon | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Direct target specificity of E2 | - |
dc.subject.keywordAuthor | p27-E2 fusion protein | - |
dc.subject.keywordAuthor | Processive ubiquitination | - |
dc.subject.keywordAuthor | Ubiquitin | - |
dc.subject.keywordAuthor | Ubiquitin-linkage | - |
dc.subject.keywordPlus | LIGASE COMPLEX | - |
dc.subject.keywordPlus | ENZYME CDC34 | - |
dc.subject.keywordPlus | CHAIN | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | P27(KIP1) | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | REPAIR | - |
dc.subject.keywordPlus | SUMO | - |
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