Lysobacter concretionis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor

Abstract

The taxonomic positions of Lysobacter species with validly published names and a novel strain Ko07<SUP>T, which was newly isolated from an upflow anaerobic sludge blanket reactor treating wastewater from a brewery, were (re)estimated on the basis of results obtained by using a polyphasic taxonomy approach. Phylogenetic inference based on 16S rRNA gene sequences showed that strain Ko07T and all Lysobacter species with validly published names clustered together in a phylogenetic branch within the class 'Gammaproteobacteria'. The sequence similarity of strain Ko07T to the type strains of established Lysobacter species was in the range 94.9-96.7%. Ubiquinone Q-8 and branched fatty acids, C</SUP><INF>11:0</INF> <SUP>iso, C</SUP><INF>15:0</INF> <SUP>iso, C</SUP><INF>16:0</INF> <SUP>iso, iso C</SUP><INF>17:1</INF> <SUP>w9c and C</SUP><INF>11:0</INF> iso 3OH, predominantly appeared in strain Ko07T as well as in all type strains of the recognized Lysobacter species. The DNA-DNA hybridization values of strain Ko07T with those of recognized Lysobacter species were estimated to be 2-20 %. Despite sharing common taxonomic features in important phenotypic characteristics, such as gliding movement, long-rod shape and proteolytic activity, strain Ko07T could be distinguished from the Lysobacter species with validly published names by its low DNA-DNA hybridization value, a comparatively low DNA G + C content (63.8 mol%), substrate utilization and some physiochemical characteristics. On the basis of the results obtained in this study, it is proposed that strain Ko07T should be classified as representing a novel member of the genus Lysobacter, for which the name Lysobacter concretionis sp. nov. is proposed. The type strain is Ko07T (= KCTC 12205T = DSM 16239T).