DC Field | Value | Language |
---|---|---|
dc.contributor.author | Heo, Won Do | ko |
dc.contributor.author | Takanari Inoue | ko |
dc.contributor.author | Wei Sun Park | ko |
dc.contributor.author | Man Lyang Kim | ko |
dc.contributor.author | Byung Ouk Park | ko |
dc.contributor.author | Thomas J. Wandless | ko |
dc.contributor.author | Tobias Meyer | ko |
dc.date.accessioned | 2013-03-06T23:21:28Z | - |
dc.date.available | 2013-03-06T23:21:28Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2006-12 | - |
dc.identifier.citation | SCIENCE, v.314, pp.1458 - 1461 | - |
dc.identifier.issn | 0036-8075 | - |
dc.identifier.uri | http://hdl.handle.net/10203/88795 | - |
dc.description.abstract | Many signaling, cytoskeletal, and transport proteins have to be localized to the plasma membrane ( PM) in order to carry out their function. We surveyed PM-targeting mechanisms by imaging the subcellular localization of 125 fluorescent protein - conjugated Ras, Rab, Arf, and Rho proteins. Out of 48 proteins that were PM-localized, 37 contained clusters of positively charged amino acids. To test whether these polybasic clusters bind negatively charged phosphatidylinositol 4,5-bisphosphate [PI(4,5) P-2] lipids, we developed a chemical phosphatase activation method to deplete PM PI(4,5) P-2. Unexpectedly, proteins with polybasic clusters dissociated from the PM only when both PI(4,5) P-2 and phosphatidylinositol 3,4,5-trisphosphate [PI( 3,4,5) P-3] were depleted, arguing that both lipid second messengers jointly regulate PM targeting. | - |
dc.language | English | - |
dc.publisher | AMER ASSOC ADVANCEMENT SCIENCE | - |
dc.title | PI(3,4,5)P-3 and PI(4,5)P-2 lipids target proteins with polybasic clusters to the plasma membrane | - |
dc.type | Article | - |
dc.identifier.wosid | 000242406100043 | - |
dc.identifier.scopusid | 2-s2.0-33845313646 | - |
dc.type.rims | ART | - |
dc.citation.volume | 314 | - |
dc.citation.beginningpage | 1458 | - |
dc.citation.endingpage | 1461 | - |
dc.citation.publicationname | SCIENCE | - |
dc.identifier.doi | 10.1126/science.1134389 | - |
dc.contributor.localauthor | Heo, Won Do | - |
dc.contributor.nonIdAuthor | Takanari Inoue | - |
dc.contributor.nonIdAuthor | Wei Sun Park | - |
dc.contributor.nonIdAuthor | Man Lyang Kim | - |
dc.contributor.nonIdAuthor | Byung Ouk Park | - |
dc.contributor.nonIdAuthor | Thomas J. Wandless | - |
dc.contributor.nonIdAuthor | Tobias Meyer | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | RAS PROTEINS | - |
dc.subject.keywordPlus | LOCALIZATION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | TRANSLOCATION | - |
dc.subject.keywordPlus | ORGANIZATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | GTPASES | - |
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