DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeong, KW | ko |
dc.contributor.author | Kim, HZ | ko |
dc.contributor.author | Kim, S | ko |
dc.contributor.author | Kim, YS | ko |
dc.contributor.author | Choe, Joonho | ko |
dc.date.accessioned | 2013-03-06T21:29:12Z | - |
dc.date.available | 2013-03-06T21:29:12Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007-01 | - |
dc.identifier.citation | ONCOGENE, v.26, no.4, pp.487 - 499 | - |
dc.identifier.issn | 0950-9232 | - |
dc.identifier.uri | http://hdl.handle.net/10203/88525 | - |
dc.description.abstract | The PDZ proteins such as hDLG, hScrib and MAGIs function as the membrane-associated protein scaffolds and have been shown to interact with the high-risk human papillomavirus (HPV) E6s. In this report, we identify a Golgi-associated PDZ protein, cystic fibrosis transmembrane regulator-associated ligand (CAL) as a cellular target of HPV16 E6 by the proteomic approach. The carboxy-terminal PDZ-binding motif of HPV16 E6 specifically interacts with the PDZ domain of CAL, and the interaction enhances proteasome-mediated degradation of CAL. HPV16 E6 interacts with CAL more strongly and degrades it better than HPV18 E6 owing to the more compatible PDZ-binding motif. CAL is ubiquitinated by the E6/E6-associated protein (E6AP) complex or by E6AP alone, albeit less efficiently, which indicates that it could be a normal target of E6AP. Although it downregulates CAL at the transcript level, small interfering RNA-induced depletion of HPV16 E6 in Caski cells stabilizes CAL at the protein level, suggesting that HPV16 E6 mediates the proteasomal degradation of CAL in HPV-positive cervical cancer cells. HPV16 E6 may tightly regulate the vesicular trafficking processes by interacting with CAL, and such a modification can contribute to the development of cervical cancer. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | CERVICAL-CANCER CELLS | - |
dc.subject | GOLGI PROTEIN | - |
dc.subject | ADENOVIRUS E4-ORF1 | - |
dc.subject | EARLY GENES | - |
dc.subject | HPV-18 E6 | - |
dc.subject | IN-VITRO | - |
dc.subject | P53 | - |
dc.subject | IDENTIFICATION | - |
dc.subject | BINDING | - |
dc.subject | ONCOPROTEINS | - |
dc.title | Human papillomavirus type 16 E6 protein interacts with cystic fibrosis transmembrane regulator-associated ligand and promotes E6-associated protein-mediated ubiquitination and proteasomal degradation | - |
dc.type | Article | - |
dc.identifier.wosid | 000243731600002 | - |
dc.identifier.scopusid | 2-s2.0-33846495746 | - |
dc.type.rims | ART | - |
dc.citation.volume | 26 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 487 | - |
dc.citation.endingpage | 499 | - |
dc.citation.publicationname | ONCOGENE | - |
dc.identifier.doi | 10.1038/sj.onc.1209837 | - |
dc.contributor.localauthor | Choe, Joonho | - |
dc.contributor.nonIdAuthor | Jeong, KW | - |
dc.contributor.nonIdAuthor | Kim, HZ | - |
dc.contributor.nonIdAuthor | Kim, S | - |
dc.contributor.nonIdAuthor | Kim, YS | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | HPV | - |
dc.subject.keywordAuthor | E6 | - |
dc.subject.keywordAuthor | E6AP | - |
dc.subject.keywordAuthor | proteasome | - |
dc.subject.keywordAuthor | ubiquitination | - |
dc.subject.keywordPlus | CERVICAL-CANCER CELLS | - |
dc.subject.keywordPlus | GOLGI PROTEIN | - |
dc.subject.keywordPlus | ADENOVIRUS E4-ORF1 | - |
dc.subject.keywordPlus | EARLY GENES | - |
dc.subject.keywordPlus | HPV-18 E6 | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | P53 | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | ONCOPROTEINS | - |
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