DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Byung-Kwan | ko |
dc.contributor.author | Knight, Eric M. | ko |
dc.contributor.author | Palsson, Bernhard O. | ko |
dc.date.accessioned | 2013-03-06T20:03:37Z | - |
dc.date.available | 2013-03-06T20:03:37Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | METHODS IN MOLECULAR BIOLOGY, v.439, no.0, pp.131 - 145 | - |
dc.identifier.issn | 1064-3745 | - |
dc.identifier.uri | http://hdl.handle.net/10203/88269 | - |
dc.description.abstract | Interactions between cis-acting elements and proteins play a key role in transcriptional regulation of all known organisms. To better understand these interactions, researchers developed a method that couples chromatin immunoprecipitation with microarrays (also known as ChIP-chip), which is capable of providing a whole-genome map of protein-DNA interactions. This versatile and high-throughput strategy is initiated by formaldehyde-mediated cross-linking of DNA and proteins, followed by cell lysis, DNA fragmentation, and immunopurification. The immunoprecipitated DNA fragments are then purified from the proteins by reverse-cross-linking followed by amplification, labeling, and hybridization to a whole-genome tiling microarray against a reference sample. The enriched signals obtained from the microarray then are normalized by the reference sample and used to generate the whole-genome map of protein-DNA interactions. The protocol described here has been used for discovering the genomewide distribution of RNA polymerase and several transcription factors of Escherichia coli. ? 2008 Humana Press. | - |
dc.language | English | - |
dc.publisher | Humana Press, Inc. | - |
dc.title | Genomewide idenqtification of protein binding locations using chromatin immunoprecipitation coupled with microarray | - |
dc.type | Article | - |
dc.identifier.scopusid | 2-s2.0-84934439157 | - |
dc.type.rims | ART | - |
dc.citation.volume | 439 | - |
dc.citation.issue | 0 | - |
dc.citation.beginningpage | 131 | - |
dc.citation.endingpage | 145 | - |
dc.citation.publicationname | METHODS IN MOLECULAR BIOLOGY | - |
dc.contributor.localauthor | Cho, Byung-Kwan | - |
dc.contributor.nonIdAuthor | Knight, Eric M. | - |
dc.contributor.nonIdAuthor | Palsson, Bernhard O. | - |
dc.description.isOpenAccess | N | - |
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