DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park K. | ko |
dc.contributor.author | Kwak I.-S. | ko |
dc.date.accessioned | 2013-03-06T16:35:34Z | - |
dc.date.available | 2013-03-06T16:35:34Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v.151, no.3, pp.349 - 354 | - |
dc.identifier.issn | 1096-4959 | - |
dc.identifier.uri | http://hdl.handle.net/10203/87614 | - |
dc.description.abstract | Environmental stressors can induce changes in gene expression that can be useful as biomarkers. To identify potential biomarkers of water quality, we characterized full-length cDNA sequences of the serine-type endopeptidase (SP) gene from Chironomus riparius. Their expression was analyzed during different life-history stages and in response to treatment with various concentrations of di(2-ethylhexyl) phthalate (DEHP) for short and long periods of time. A comparative molecular and phylogenetic investigation was then conducted among different orders of insects using sequence database analysis. The sequence of the C riparius SP gene was found to be most closely related to the sequence of SPs isolated from Aedes aegypti. In addition, the basal level of C riparius SP mRNA was more highly expressed in larvae than in other life-history stages. However, the expression of C riparius SP was primarily limited to the gut in larvae. When the effects of short-term exposure to DEHP were evaluated, C riparius SP gene expression decreased within 1 h of treatment, regardless of dose. We also investigated expression of the C riparius SP gene following long-term DEHP exposure (10 days) and found that it decreased significantly across all DEHP dosages. Finally, the response of the SP gene was more sensitive in C riparius that were exposed to low concentrations of DEHP than in those that were exposed to high concentrations. These results show that suppression of the C riparius SP gene by DEHP is as a potential biomarker that could be useful for monitoring aquatic quality. (C) 2008 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.subject | DI(2-ETHYLHEXYL)PHTHALATE DEHP | - |
dc.subject | MAJOR ALLERGEN | - |
dc.subject | PROTEASES | - |
dc.subject | IDENTIFICATION | - |
dc.subject | CHYMOTRYPSIN | - |
dc.subject | PHTHALATE | - |
dc.subject | LARVAE | - |
dc.subject | CELLS | - |
dc.subject | ENZYMES | - |
dc.subject | PLASTICIZERS | - |
dc.title | Expression of Chironomus riparius serine-type endopeptidase gene under di-(2-ethylhexyl)-phthalate (DEHP) exposure | - |
dc.type | Article | - |
dc.identifier.wosid | 000260819900015 | - |
dc.identifier.scopusid | 2-s2.0-52149124352 | - |
dc.type.rims | ART | - |
dc.citation.volume | 151 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 349 | - |
dc.citation.endingpage | 354 | - |
dc.citation.publicationname | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | - |
dc.identifier.doi | 10.1016/j.cbpb.2008.08.004 | - |
dc.contributor.nonIdAuthor | Kwak I.-S. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Chironomus riparius | - |
dc.subject.keywordAuthor | Serine-type endopeptidase | - |
dc.subject.keywordAuthor | DEHP | - |
dc.subject.keywordAuthor | Biomarker | - |
dc.subject.keywordAuthor | Aquatic biomonitoring | - |
dc.subject.keywordPlus | DI(2-ETHYLHEXYL)PHTHALATE DEHP | - |
dc.subject.keywordPlus | MAJOR ALLERGEN | - |
dc.subject.keywordPlus | PROTEASES | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | CHYMOTRYPSIN | - |
dc.subject.keywordPlus | PHTHALATE | - |
dc.subject.keywordPlus | LARVAE | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | ENZYMES | - |
dc.subject.keywordPlus | PLASTICIZERS | - |
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