Paenibacillus donghaensis sp nov., a xylan-degrading and nitrogen-fixing bacterium isolated from East Sea sediment

Abstract

A Gram-positive and endospore-forming strain, JH8(T), was isolated from deep-sea sediment and identified as a member of the genus Paenibacillus on the basis of 16S rRNA gene sequence and phenotypic analyses. According to a phylogenetic analysis, the most closely related species was Paenibacillus wynnii LMG 22176(T) (96.9%). Strain JH8(T) was also facultatively anaerobic and grew optimally at 20-25 degrees C. The major cellular fatty acid was anteiso-C-15:0, and the DNA G+C content was 53.1 mol%. The DNA-DNA relatedness between the isolate and Paenibacillus wynnii LMG 22176(T) was 7.6%, indicating that strain JH8(T) and P wynnii belong to different species. Based on the phylogenetic, phenotypic, and chemotaxonomic characteristics, strain JH8(T) would appear to belong to a novel species, for which the name Paenibacillus donghaensis sp. nov. is proposed (type strain=KCTC 13049(T) =LMG 23780(T)).