Identification of marker, compounds for discriminating between embryogenic and nonembryogenic calluses of higher plants using pyrolysis gas chromatography mass spectrometry and genetic programming

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When whole cells are subjected to pyrolysis gas chromatography/mass spectrometry (Py-GC/MS) analysis, it provides biochemical profiles containing overlapping signals of the majority of compounds. To determine marker compounds that discriminate embryogenic calluses from nonembryogenic calluses, samples of embryogenic and nonembryogenic calluses of five higher plant species were subjected to Py-GC/MS. Genetic programming of Py-GC/MS data was able to discriminate embryogenic calluses from nonembryogenic calluses. The content ratio of 5-meyhyl-2-furancarboxaldehyde and 5-(hydroxymethyl)-2-furancarboxaldehyde was greater in nonembryogenic calluses than in embryogenic calluses. However, the content ratio of phenol, p-cresol, and 'H-indole in embryogenic calluses was 1.2 to 2.4 times greater than the ratio in nonembryogenic calluses. These pyrolysates seem to be derived from the components of the cell walls, which suggests that differences in cell wall components or changes in the architecture of the cell wall play a crucial role in determining the embryogenic competence of calluses.
Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Issue Date
2006-01
Language
English
Article Type
Article
Keywords

CELL-SUSPENSION CULTURES; SOMATIC EMBRYOGENESIS; REGENERATION; GINKGOLIDES; BILOBALIDE; PROTEINS; STRESS; GROWTH

Citation

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.11, no.1, pp.38 - 42

ISSN
1226-8372
DOI
10.1007/BF02931866
URI
http://hdl.handle.net/10203/86406
Appears in Collection
MSE-Journal Papers(저널논문)
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