DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, Eui-Cheol | ko |
dc.contributor.author | Shin, WC | ko |
dc.contributor.author | Choi, Y | ko |
dc.contributor.author | Kim, H | ko |
dc.contributor.author | Park, JH | ko |
dc.contributor.author | Kim, SJ | ko |
dc.date.accessioned | 2013-03-06T03:55:46Z | - |
dc.date.available | 2013-03-06T03:55:46Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2001-03 | - |
dc.identifier.citation | CANCER IMMUNOLOGY IMMUNOTHERAPY, v.50, no.1, pp.23 - 30 | - |
dc.identifier.issn | 0340-7004 | - |
dc.identifier.uri | http://hdl.handle.net/10203/85733 | - |
dc.description.abstract | Many tumors, including hepatocellular carcinomas (HCCs), resist Fas-mediated cell death, which is one of the effector mechanisms in the host's anti-tumor response; however, this resistance can be abolished by interferon-gamma (IFN-gamma). IFN-gamma may sensitize Fas-mediated cell death in several ways, but the exact mechanism in HCCs is uncertain. In this study, we thoroughly investigated the effect of IFN-gamma on the susceptibility of one human normal liver cell line and 12 HCC cell lines to Fas-mediated cell death. We also investigated the effect of IFN-gamma on the expression of various apoptosis-related genes such. as the Fas/TNF-related genes, the bcl-2 family, and the caspase family of genes. Although most cell lines showed considerable constitutive expression of Fas, all tested cell lines resisted Fas-mediated cell death without IFN-gamma. When cells were pretreated with IFN-gamma, only three cell lines were made significantly susceptible to Fas-mediated cell death (SNU-354, SNU-387 and SNU-423); the other 10 cell lines were not affected. IFN-gamma increased the mRNA expression of Fas, TRAIL and caspase-1, and surface Fas was also increased. The strongly sensitized cell lines (SNU-354, SNU-387 and SNU-423) showed a particularly potent increment in surface Fas after IFN-gamma treatment (increase in surface Fas > 1.7-fold). This result enabled us to conclude that a potent increment of surface Fas expression is a major sensitizing mechanism of IFN-gamma. We conclude that IFN gamma cannot play a sensitizing role in most HCC cell lines and that IFN-gamma makes HCC cells susceptible to Fas-mediated cell death through a marked up-regulation of surface Fas in some HCC cells. | - |
dc.language | English | - |
dc.publisher | SPRINGER-VERLAG | - |
dc.subject | HUMAN HEPATOCELLULAR-CARCINOMA | - |
dc.subject | MEDIATED APOPTOSIS | - |
dc.subject | TNF-ALPHA | - |
dc.subject | IFN-GAMMA | - |
dc.subject | SURFACE ANTIGEN | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | UP-REGULATION | - |
dc.subject | LIGAND | - |
dc.subject | LINES | - |
dc.subject | RESISTANCE | - |
dc.title | Effect of interferon-gamma on the susceptibility to Fas (CD95/APO-1)-mediated cell death in human hepatoma cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000167640500004 | - |
dc.identifier.scopusid | 2-s2.0-0035088926 | - |
dc.type.rims | ART | - |
dc.citation.volume | 50 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 23 | - |
dc.citation.endingpage | 30 | - |
dc.citation.publicationname | CANCER IMMUNOLOGY IMMUNOTHERAPY | - |
dc.identifier.doi | 10.1007/s002620000166 | - |
dc.contributor.localauthor | Shin, Eui-Cheol | - |
dc.contributor.nonIdAuthor | Shin, WC | - |
dc.contributor.nonIdAuthor | Choi, Y | - |
dc.contributor.nonIdAuthor | Kim, H | - |
dc.contributor.nonIdAuthor | Park, JH | - |
dc.contributor.nonIdAuthor | Kim, SJ | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | IFN-gamma | - |
dc.subject.keywordAuthor | Fas | - |
dc.subject.keywordAuthor | cell death | - |
dc.subject.keywordAuthor | hepatoma | - |
dc.subject.keywordPlus | HUMAN HEPATOCELLULAR-CARCINOMA | - |
dc.subject.keywordPlus | MEDIATED APOPTOSIS | - |
dc.subject.keywordPlus | TNF-ALPHA | - |
dc.subject.keywordPlus | IFN-GAMMA | - |
dc.subject.keywordPlus | SURFACE ANTIGEN | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | UP-REGULATION | - |
dc.subject.keywordPlus | LIGAND | - |
dc.subject.keywordPlus | LINES | - |
dc.subject.keywordPlus | RESISTANCE | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.