DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sung, YH | ko |
dc.contributor.author | Song, YJ | ko |
dc.contributor.author | Lim, SW | ko |
dc.contributor.author | Chung, JY | ko |
dc.contributor.author | Lee, Gyun-Min | ko |
dc.date.accessioned | 2013-03-05T03:52:06Z | - |
dc.date.available | 2013-03-05T03:52:06Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2004-09 | - |
dc.identifier.citation | JOURNAL OF BIOTECHNOLOGY, v.112, pp.323 - 335 | - |
dc.identifier.issn | 0168-1656 | - |
dc.identifier.uri | http://hdl.handle.net/10203/85356 | - |
dc.description.abstract | Human thrombopoietin (hTPO) is a heavily glycosylated protein with 6 and 24 potential N- and O-glycosylation sites, respectively. To determine the effect of sodium butyrate (NaBu) on the production and quality of hTPO in recombinant Chinese hamster ovary (rCHO) cells, NaBu (0-10 mM) was added to the cultures of exponentially growing cells. NaBu addition significantly increased both the specific and volumetric hTPO production, although it decreased the cell viability by apoptosis in a dose-dependent manner. The highest hTPO concentration of 82.2 +/- 5.6 mug ml(-1) was obtained in the culture with 3 mM NaBu addition. Compared with the culture without NaBu addition, the culture with 3 mM NaBu resulted in a 6.4-fold increase in q(TPO) and a 3.3-fold increase in the final hTPO concentration on day 7. However, NaBu deteriorated the quality of hTPO, resulting from increased heterogeneity, reduced acidic hTPO isoforms, reduced alpha(2 --> 3) sialylation, and decreased in vivo biological activity. We also found that the biological activity of hTPO in the culture with 3 mM NaBu addition collected on day 7 was 72% of that in the culture without NaBu addition. Taken together, the use of NaBu or its optimal concentration for high-level expression of a heavily glycosylated protein like hTPO should be determined by considering its detrimental effect on the quality of glycoprotein. (C) 2004 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | HIGH-LEVEL EXPRESSION | - |
dc.subject | MEGAKARYOCYTE GROWTH | - |
dc.subject | CULTURE SUPERNATANT | - |
dc.subject | ANTIBODY-PRODUCTION | - |
dc.subject | INDUCED APOPTOSIS | - |
dc.subject | GLYCOSYLATION | - |
dc.subject | PROTEINS | - |
dc.subject | SIALYLTRANSFERASE | - |
dc.subject | THROMBOCYTOPENIA | - |
dc.subject | BIOSYNTHESIS | - |
dc.title | Effect of sodium butyrate on the production, heterogeneity and biological activity of human thrombopoietin by recombinant Chinese hamster ovary cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000223649700009 | - |
dc.identifier.scopusid | 2-s2.0-4143145162 | - |
dc.type.rims | ART | - |
dc.citation.volume | 112 | - |
dc.citation.beginningpage | 323 | - |
dc.citation.endingpage | 335 | - |
dc.citation.publicationname | JOURNAL OF BIOTECHNOLOGY | - |
dc.identifier.doi | 10.1016/j.jbiotec.2004.05.003 | - |
dc.contributor.localauthor | Lee, Gyun-Min | - |
dc.contributor.nonIdAuthor | Sung, YH | - |
dc.contributor.nonIdAuthor | Song, YJ | - |
dc.contributor.nonIdAuthor | Lim, SW | - |
dc.contributor.nonIdAuthor | Chung, JY | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | biological activity | - |
dc.subject.keywordAuthor | CHO cells | - |
dc.subject.keywordAuthor | human thrombopoietin | - |
dc.subject.keywordAuthor | salic acid | - |
dc.subject.keywordAuthor | sodium butyrate | - |
dc.subject.keywordPlus | HIGH-LEVEL EXPRESSION | - |
dc.subject.keywordPlus | MEGAKARYOCYTE GROWTH | - |
dc.subject.keywordPlus | CULTURE SUPERNATANT | - |
dc.subject.keywordPlus | ANTIBODY-PRODUCTION | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | GLYCOSYLATION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | SIALYLTRANSFERASE | - |
dc.subject.keywordPlus | THROMBOCYTOPENIA | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
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