DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoon, SK | ko |
dc.contributor.author | Hong, JK | ko |
dc.contributor.author | Lee, Gyun-Min | ko |
dc.date.accessioned | 2013-03-05T03:47:36Z | - |
dc.date.available | 2013-03-05T03:47:36Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2004-07 | - |
dc.identifier.citation | BIOTECHNOLOGY PROGRESS, v.20, pp.1293 - 1296 | - |
dc.identifier.issn | 8756-7938 | - |
dc.identifier.uri | http://hdl.handle.net/10203/85340 | - |
dc.description.abstract | A single stressful culture condition induced by hypoosmotic stress (210 mOsm kg(-1)), low culture temperature (32 degreesC), or NaBu addition (1 mM) resulted in a 1.8- to 2.2-fold enhancement of specific erythropoietin (EPO) productivity (q(EPO)) of recombinant Chinese hamster ovary (rCHO) cells compared to normal culture condition (37 degreesC and 310 mOsm kg(-1)). Simultaneous application of these stressful conditions further enhanced qEPO up to approximately 5-fold. However, the quality of EPO was affected by stressful culture conditions. The proportion of acidic isoforms of EPO under a single stressful condition was 2.8-13.8% lower than that under normal culture condition. Simultaneous application of the stressful conditions further decreased the portion of acidic isoforms but not significantly. Despite 5-fold enhancement of q(EPO), the portion of acidic isoforms under the simultaneous application of stressful culture conditions was 12.9-21.6% lower than that under normal culture condition. Taken together, these results suggest the potential of simultaneous application of different stressful culture conditions to the production phase of two-stage culture, where cell growth and production phases are separated, for improved EPO production. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | RECOMBINANT CHO CELLS | - |
dc.subject | ANTIBODY-PRODUCTION | - |
dc.subject | SODIUM-BUTYRATE | - |
dc.subject | LOW-TEMPERATURE | - |
dc.subject | T-PA | - |
dc.subject | GLYCOSYLATION | - |
dc.subject | EXPRESSION | - |
dc.subject | PRESSURE | - |
dc.subject | GROWTH | - |
dc.title | Effect of simultaneous application of stressful culture conditions on specific productivity and heterogeneity of erythropoietin in chinese hamster ovary cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000223181000043 | - |
dc.identifier.scopusid | 2-s2.0-4043105472 | - |
dc.type.rims | ART | - |
dc.citation.volume | 20 | - |
dc.citation.beginningpage | 1293 | - |
dc.citation.endingpage | 1296 | - |
dc.citation.publicationname | BIOTECHNOLOGY PROGRESS | - |
dc.identifier.doi | 10.1021/bp034382z | - |
dc.contributor.localauthor | Lee, Gyun-Min | - |
dc.contributor.nonIdAuthor | Yoon, SK | - |
dc.contributor.nonIdAuthor | Hong, JK | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | RECOMBINANT CHO CELLS | - |
dc.subject.keywordPlus | ANTIBODY-PRODUCTION | - |
dc.subject.keywordPlus | SODIUM-BUTYRATE | - |
dc.subject.keywordPlus | LOW-TEMPERATURE | - |
dc.subject.keywordPlus | T-PA | - |
dc.subject.keywordPlus | GLYCOSYLATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PRESSURE | - |
dc.subject.keywordPlus | GROWTH | - |
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