Continuous culture of immobilized Streptomyces cells for kasugamycin production

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Continuous cultures of immobilized Streptomyces kasugaensis, a kasugamycin producer, were carried out an Celite beads. When using a prototype separator for immobilized-cell separation and recycling, the continuous operation could not be sustained for an extended period as a result of an excessive loss ofimmobilized cells caused by the poor performance of the separator. Accordingly, the immobilized-cell separator was revised to provide better immobilized-cell settling and thus recycling into the reactor. In a subsequent culture using the revised separator, a stable operation was maintained for over 820 h with a high kasugamycin productivity. The kasugamycin productivity ranged from 9.8 to 16.1 mg/L/h, which was about 14- to 23-fold higher than that in a batch suspended-cell culture. When the original feeding medium concentration was doubled at the end of the continuous culture, the productivity became severely impaired for several reasons, which will be discussed. An excessive formation of free cells and loss of immobilized cells through the separator were also observed.
Publisher
AMER CHEMICAL SOC
Issue Date
2001-05
Language
English
Article Type
Article
Keywords

FLUIDIZED-BED BIOREACTOR; CONFINING MYCELIAL GROWTH; CALCIUM ALGINATE BEADS; PENICILLIUM-CHRYSOGENUM; TOLYPOCLADIUM-INFLATUM; ANTIBIOTIC PRODUCTION; COELICOLOR A3(2); CYCLOSPORINE-A; BUBBLE COLUMN; FERMENTATION

Citation

BIOTECHNOLOGY PROGRESS, v.17, no.3, pp.453 - 461

ISSN
8756-7938
URI
http://hdl.handle.net/10203/85278
Appears in Collection
CBE-Journal Papers(저널논문)
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