DC Field | Value | Language |
---|---|---|
dc.contributor.author | One Choi | ko |
dc.contributor.author | Noboru Tomiya | ko |
dc.contributor.author | Kim, Jung Hoe | ko |
dc.contributor.author | James M. Slavicek | ko |
dc.contributor.author | Michael J. Betenbaugh | ko |
dc.contributor.author | Yuan C. Lee | ko |
dc.date.accessioned | 2013-03-05T02:37:56Z | - |
dc.date.available | 2013-03-05T02:37:56Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2003-07 | - |
dc.identifier.citation | GLYCOBIOLOGY, v.13, pp.539 - 548 | - |
dc.identifier.issn | 0959-6658 | - |
dc.identifier.uri | http://hdl.handle.net/10203/85082 | - |
dc.description.abstract | N-glycan structures of recombinant human serum transferrin (hTf) expressed by Lymantria dispar (gypsy moth) 652Y cells were determined. The gene encoding hTf was incorporated into a Lymantria dispar nucleopolyhedrovirus (LdMNPV) under the control of the polyhedrin promoter. This virus was then used to infect Ld652Y cells, and the recombinant protein was harvested at 120 h postinfection. N-glycans were released from the purified recombinant human serum transferrin and derivatized with 2-aminopyridine; the glycan structures were analyzed by a two-dimensional HPLC and MALDI-TOF MS. Structures of 11 glycans (88.8% of total N-glycans) were elucidated. The glycan analysis revealed that the most abundant glycans were Man(1-3)(+/- Fucalpha6)GlcNAc(2) (75.5%) and GlcNAcMan(3)( +/- Fucalpha6)GlcNAc(2) (7.4%). There was only similar to6% of high-mannose type glycans identified. Nearly half (49.8%) of the total N-glycans contained alpha(1,3)-fucosylation on the Asn-linked GlcNAc residue. However alpha(1,3)-fucosylation on the same GlcNAc, often found in N-glycans produced by other insects and insect cells, was not detected. Inclusion of fetal bovine serum in culture media had little effect on the N-glycan structures of the recombinant human serum transferrin obtained. | - |
dc.language | English | - |
dc.publisher | OXFORD UNIV PRESS INC | - |
dc.subject | NUCLEAR POLYHEDROSIS-VIRUS | - |
dc.subject | 2-DIMENSIONAL SUGAR MAP | - |
dc.subject | INSECT CELLS | - |
dc.subject | LINKED OLIGOSACCHARIDES | - |
dc.subject | MAMMALIAN BETA-1,4-GALACTOSYLTRANSFERASE | - |
dc.subject | RECOMBINANT PROTEINS | - |
dc.subject | MEMBRANE-GLYCOPROTEINS | - |
dc.subject | GLYCOSYLATION PATHWAY | - |
dc.subject | BACULOVIRUS VECTOR | - |
dc.subject | IDENTIFICATION | - |
dc.title | N-glycan structures of human transferrin produced by Lymantria dispar (gypsy moth) cells using the LdMNPV expression system | - |
dc.type | Article | - |
dc.identifier.wosid | 000183724600005 | - |
dc.identifier.scopusid | 2-s2.0-0037819506 | - |
dc.type.rims | ART | - |
dc.citation.volume | 13 | - |
dc.citation.beginningpage | 539 | - |
dc.citation.endingpage | 548 | - |
dc.citation.publicationname | GLYCOBIOLOGY | - |
dc.identifier.doi | 10.1093/glycob/cwg071 | - |
dc.contributor.localauthor | Kim, Jung Hoe | - |
dc.contributor.nonIdAuthor | One Choi | - |
dc.contributor.nonIdAuthor | Noboru Tomiya | - |
dc.contributor.nonIdAuthor | James M. Slavicek | - |
dc.contributor.nonIdAuthor | Michael J. Betenbaugh | - |
dc.contributor.nonIdAuthor | Yuan C. Lee | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | baculovirus | - |
dc.subject.keywordAuthor | gypsy moth | - |
dc.subject.keywordAuthor | insect cells | - |
dc.subject.keywordAuthor | Lymantria dispar nucleopolyhedrovirus | - |
dc.subject.keywordAuthor | N-glycan | - |
dc.subject.keywordPlus | NUCLEAR POLYHEDROSIS-VIRUS | - |
dc.subject.keywordPlus | 2-DIMENSIONAL SUGAR MAP | - |
dc.subject.keywordPlus | INSECT CELLS | - |
dc.subject.keywordPlus | LINKED OLIGOSACCHARIDES | - |
dc.subject.keywordPlus | MAMMALIAN BETA-1,4-GALACTOSYLTRANSFERASE | - |
dc.subject.keywordPlus | RECOMBINANT PROTEINS | - |
dc.subject.keywordPlus | MEMBRANE-GLYCOPROTEINS | - |
dc.subject.keywordPlus | GLYCOSYLATION PATHWAY | - |
dc.subject.keywordPlus | BACULOVIRUS VECTOR | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
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