Fission yeast Dna2 is required for generation of the telomeric single-strand overhang

Cited 58 time in webofscience Cited 0 time in scopus
  • Hit : 326
  • Download : 0
DC FieldValueLanguage
dc.contributor.authorTomita, Kazunoriko
dc.contributor.authorKibe, Tatsuyako
dc.contributor.authorKang, Ho-Youngko
dc.contributor.authorSeo, Yeon-Sooko
dc.contributor.authorUritani, Masahiroko
dc.contributor.authorUshimaru, Takashiko
dc.contributor.authorUeno, Masaruko
dc.date.accessioned2013-03-04T15:21:06Z-
dc.date.available2013-03-04T15:21:06Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2004-11-
dc.identifier.citationMOLECULAR AND CELLULAR BIOLOGY, v.24, no.21, pp.9557 - 9567-
dc.identifier.issn0270-7306-
dc.identifier.urihttp://hdl.handle.net/10203/83053-
dc.description.abstractIt has been suggested that the Schizosaccharomyces pombe Rad50 (Rad50-Rad32-Nbs1) complex is required for the resection of the C-rich strand at telomere ends in taz1-d cells. However, the nuclease-deficient Rad32-D25A mutant can still resect the C-rich strand, suggesting the existence of a nuclease that resects the C-rich strand. Here, we demonstrate that a taz1-d dna2-2C double mutant lost the G-rich overhang at a semipermissive temperature. The amount of G-rich overhang in S phase in the dna2-C2 mutant was lower than that in wild-type cells at the semipermissive temperature. Dna2 bound to telomere DNA in a chromatin immunoprecipitation assay. Moreover, telomere length decreased with each generation after shift of the dna2-2C mutant to the semipermissive temperature. These results suggest that Dna2 is involved in the generation of G-rich overhangs in both wild-type cells and taz1-d cells. The dna2-C2 mutant was not gamma ray sensitive at the semipermissive temperature, suggesting that the ability to process double-strand break (DSB) ends was not affected in the dna2-C2 mutant. Our results reveal that DSB ends and telomere ends are processed by different mechanisms.-
dc.languageEnglish-
dc.publisherAMER SOC MICROBIOLOGY-
dc.subjectOKAZAKI FRAGMENT MATURATION-
dc.subjectSCHIZOSACCHAROMYCES-POMBE-
dc.subjectSACCHAROMYCES-CEREVISIAE-
dc.subjectPOLYMERASE ALPHA-
dc.subjectS-PHASE-
dc.subjectCATALYTIC SUBUNIT-
dc.subjectKU HETERODIMER-
dc.subjectBUDDING YEAST-
dc.subjectG TAILS-
dc.subjectREPLICATION-
dc.titleFission yeast Dna2 is required for generation of the telomeric single-strand overhang-
dc.typeArticle-
dc.identifier.wosid000224943300027-
dc.identifier.scopusid2-s2.0-6344284126-
dc.type.rimsART-
dc.citation.volume24-
dc.citation.issue21-
dc.citation.beginningpage9557-
dc.citation.endingpage9567-
dc.citation.publicationnameMOLECULAR AND CELLULAR BIOLOGY-
dc.identifier.doi10.1128/MCB.24.21.9557-9567.2004-
dc.contributor.localauthorSeo, Yeon-Soo-
dc.contributor.nonIdAuthorTomita, Kazunori-
dc.contributor.nonIdAuthorKibe, Tatsuya-
dc.contributor.nonIdAuthorKang, Ho-Young-
dc.contributor.nonIdAuthorUritani, Masahiro-
dc.contributor.nonIdAuthorUshimaru, Takashi-
dc.contributor.nonIdAuthorUeno, Masaru-
dc.type.journalArticleArticle-
dc.subject.keywordPlusOKAZAKI FRAGMENT MATURATION-
dc.subject.keywordPlusSCHIZOSACCHAROMYCES-POMBE-
dc.subject.keywordPlusSACCHAROMYCES-CEREVISIAE-
dc.subject.keywordPlusPOLYMERASE ALPHA-
dc.subject.keywordPlusS-PHASE-
dc.subject.keywordPlusCATALYTIC SUBUNIT-
dc.subject.keywordPlusKU HETERODIMER-
dc.subject.keywordPlusBUDDING YEAST-
dc.subject.keywordPlusG TAILS-
dc.subject.keywordPlusREPLICATION-
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 58 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0