DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jin Woo | ko |
dc.contributor.author | Joe, Cheol O | ko |
dc.contributor.author | Choi, Eui-Ju | ko |
dc.date.accessioned | 2013-03-04T14:54:50Z | - |
dc.date.available | 2013-03-04T14:54:50Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2001-07 | - |
dc.identifier.citation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.276, no.29, pp.27064 - 27070 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/10203/82997 | - |
dc.description.abstract | Receptor-interacting protein (RIP), a death domain serine/threonine kinase, has been Shown to play a critical role in tumor necrosis factor-alpha (TNF-alpha)-induced activation of the nuclear factor-kappaB signaling pathway. We demonstrate here that ectopically expressed RIP induces I-kappaB kinase-beta (IKK beta) activation in intact cells and that RIP-induced IKK beta activation can be blocked by a kinase-inactive form of MEKK1, MEKK1(K1253M). Interestingly, RIP physically associated with MEKK1 both in vitro and in vivo. RIP phosphorylated MEKK1 at Ser-957 and Ser-994. Our data also indicate that RIP induced the stimulation of MEKK1 but not MEKK1 (S957A/S994A) in transfected cells. Furthermore, overexpressed MEKK1 (S957A/S994A) inhibited the RIP-induced activation of both IKK beta and nuclear factor-kappaB. We also demonstrated that the TNF-alpha -induced MEKK1 activation was defective in RIP-deficient Jurkat cells. Taken together, our results suggest that RIP phosphorylates and activates MEKK1 and that RIP is involved in TNF-alpha -induced MEKK1 activation. | - |
dc.language | English | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | DOMAIN KINASE RIP | - |
dc.subject | SIGNALING COMPLEX DISC | - |
dc.subject | INDUCED CELL-DEATH | - |
dc.subject | TNF RECEPTOR | - |
dc.subject | INDUCED APOPTOSIS | - |
dc.subject | TERMINAL KINASE | - |
dc.subject | IKK ACTIVATION | - |
dc.subject | JNK | - |
dc.subject | PHOSPHORYLATION | - |
dc.title | Role of receptor-interacting protein in tumor necrosis factor-alpha-dependent MEKK1 activation | - |
dc.type | Article | - |
dc.identifier.wosid | 000169966900045 | - |
dc.identifier.scopusid | 2-s2.0-0035920178 | - |
dc.type.rims | ART | - |
dc.citation.volume | 276 | - |
dc.citation.issue | 29 | - |
dc.citation.beginningpage | 27064 | - |
dc.citation.endingpage | 27070 | - |
dc.citation.publicationname | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.contributor.localauthor | Kim, Jin Woo | - |
dc.contributor.localauthor | Joe, Cheol O | - |
dc.contributor.nonIdAuthor | Choi, Eui-Ju | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | DOMAIN KINASE RIP | - |
dc.subject.keywordPlus | SIGNALING COMPLEX DISC | - |
dc.subject.keywordPlus | INDUCED CELL-DEATH | - |
dc.subject.keywordPlus | TNF RECEPTOR | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | TERMINAL KINASE | - |
dc.subject.keywordPlus | IKK ACTIVATION | - |
dc.subject.keywordPlus | JNK | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
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