DC Field | Value | Language |
---|---|---|
dc.contributor.author | Woo, Eui-Jeon | ko |
dc.contributor.author | Kim, Yeon-Gil | ko |
dc.contributor.author | Kim, Min-Sung | ko |
dc.contributor.author | Han, Won-Deok | ko |
dc.contributor.author | Shin, Sejeong | ko |
dc.contributor.author | Robinson, Howard | ko |
dc.contributor.author | Park, Sam-Yong | ko |
dc.contributor.author | Oh, Byung-Ha | ko |
dc.date.accessioned | 2013-03-04T14:24:54Z | - |
dc.date.available | 2013-03-04T14:24:54Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2004-05 | - |
dc.identifier.citation | MOLECULAR CELL, v.14, no.4, pp.531 - 539 | - |
dc.identifier.issn | 1097-2765 | - |
dc.identifier.uri | http://hdl.handle.net/10203/82943 | - |
dc.description.abstract | CAD/DFF40 is responsible for the degradation of chromosomal DNA into nucleosomal fragments and subsequent chromatin condensation during apoptosis. It exists as an inactive complex with its inhibitor ICAD/DFF45 in proliferating cells but becomes activated upon cleavage of ICAD/DFF45 into three domains by caspases in dying cells. The molecular mechanism underlying the control and activation of CAD/DFF40 was unknown. Here, the crystal structure of activated CAD/DFF40 reveals that it is a pair of molecular scissors with a deep active-site crevice that appears ideal for distinguishing internucleosomal DNA from nucleosomal DNA. Ensuing studies show that ICAD/DFF45 sequesters the nonfunctional CAD/DFF40 monomer and is also able to disassemble the functional CAD/DFF40 dimer. This capacity requires the involvement of the middle domain of ICAD/DFF45, which by itself cannot remain bound to CAD/DFF40 due to low binding affinity for the enzyme. Thus, the consequence of the caspase-cleavage of ICAD/DFF45 is a self-assembly of CAD/DFF40 into the active dimer. | - |
dc.language | English | - |
dc.publisher | CELL PRESS | - |
dc.subject | DNA FRAGMENTATION | - |
dc.subject | CHROMATIN CONDENSATION | - |
dc.subject | ACTIVE-SITE | - |
dc.subject | INHIBITOR | - |
dc.subject | NUCLEASE | - |
dc.subject | DFF40 | - |
dc.subject | CAD | - |
dc.subject | CLEAVAGE | - |
dc.subject | PROTEIN | - |
dc.subject | DOMAIN | - |
dc.title | Structural mechanism for inactivation and activation of CAD/DFF40 in the apoptotic pathway | - |
dc.type | Article | - |
dc.identifier.wosid | 000221597000016 | - |
dc.identifier.scopusid | 2-s2.0-2442505378 | - |
dc.type.rims | ART | - |
dc.citation.volume | 14 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 531 | - |
dc.citation.endingpage | 539 | - |
dc.citation.publicationname | MOLECULAR CELL | - |
dc.identifier.doi | 10.1016/S1097-2765(04)00258-8 | - |
dc.contributor.localauthor | Oh, Byung-Ha | - |
dc.contributor.nonIdAuthor | Woo, Eui-Jeon | - |
dc.contributor.nonIdAuthor | Kim, Yeon-Gil | - |
dc.contributor.nonIdAuthor | Kim, Min-Sung | - |
dc.contributor.nonIdAuthor | Han, Won-Deok | - |
dc.contributor.nonIdAuthor | Shin, Sejeong | - |
dc.contributor.nonIdAuthor | Robinson, Howard | - |
dc.contributor.nonIdAuthor | Park, Sam-Yong | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | DNA FRAGMENTATION | - |
dc.subject.keywordPlus | CHROMATIN CONDENSATION | - |
dc.subject.keywordPlus | ACTIVE-SITE | - |
dc.subject.keywordPlus | INHIBITOR | - |
dc.subject.keywordPlus | NUCLEASE | - |
dc.subject.keywordPlus | DFF40 | - |
dc.subject.keywordPlus | CAD | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | DOMAIN | - |
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